Zhang Xiaotao, Wang Ruoning, Zhang Li, Ruan Yibin, Wang Weiwei, Ji Houwei, Lin Fucheng, Liu Jian
Technology Center, China Tobacco Guizhou Industrial Co., Ltd, Guiyang, 550009, China.
College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, 310058, China.
Rapid Commun Mass Spectrom. 2018 Oct 30;32(20):1791-1798. doi: 10.1002/rcm.8222.
The minor alkaloids in tobacco play an important role in the chemical composition of cigarette smoke, and they are precursors of tobacco-specific nitrosamines (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N-nitrosonornicotine (NNN), N-nitrosoanabasine (NAB) and N-nitrosoanatabine (NAT)). NNN and NNK are classified as group 1 carcinogens. A method quantitating both tobacco minor alkaloids and tobacco-specific nitrosamines in mainstream smoke has not been reported.
Tobacco minor alkaloids and tobacco-specific nitrosamines in cigarette mainstream smoke were extracted by sonication. The extract was cleaned up by dispersive solid-phase extraction, and separation was achieved via ultra-performance liquid chromatography/tandem orbitrap mass spectrometry.
The method was validated by analysis of six replicate samples spiked with three levels of the analyses. The mean recoveries for the six replicates were from 84.7% to 118% with less than 15% relative standard deviation except myosmine at the low spiked level and the calculated detection limits were 0.066 to 13.2 ng/cig, respectively. The mean concentrations of nicotyrine, anabasine, nornicotine, anatabine, myosmine, 2,3-bipyridine, cotinine, nicotelline, N-formylnornicotine, NNK, NNN, NAB and NAT in 30 different brands of commercial cigarette smoke under the ISO smoking regimen were 2.50 μg/cig, 2.34 μg/cig, 3.21 μg/cig, 5.78 μg/cig, 2.83 μg/cig, 1.05 μg/cig, 1.55 μg/cig, 0.55 μg/cig, 2.48 μg/cig, 6.06 ng/cig, 3.62 ng/cig, 0.40 ng/cig and 6.15 ng/cig, respectively.
The proposed method was suitable for analysis of tobacco minor alkaloids and tobacco-specific nitrosamines in cigarette mainstream smoke.
烟草中的次要生物碱在卷烟烟气的化学成分中起着重要作用，并且它们是烟草特有亚硝胺（4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁酮（NNK）、N-亚硝基降烟碱（NNN）、N-亚硝基新烟草碱（NAB）和N-亚硝基假木贼碱（NAT））的前体。NNN和NNK被归类为1类致癌物。尚未有报道一种能同时定量主流烟气中烟草次要生物碱和烟草特有亚硝胺的方法。
通过超声提取卷烟主流烟气中的烟草次要生物碱和烟草特有亚硝胺。提取物通过分散固相萃取进行净化，并通过超高效液相色谱/串联轨道阱质谱进行分离。
通过分析加标了三个水平分析物的六个重复样品对该方法进行了验证。六个重复样品的平均回收率为84.7%至118%，相对标准偏差小于15%，低加标水平下的麦斯明除外，计算出的检测限分别为0.066至13.2 ng/支。在ISO吸烟条件下，30个不同品牌商业卷烟烟气中烟碱、新烟草碱、降烟碱、假木贼碱、麦斯明、2,3-联吡啶、可替宁、去甲烟碱、N-甲酰基降烟碱、NNK、NNN、NAB和NAT的平均浓度分别为2.50 μg/支、2.34 μg/支、3.21 μg/支、5.78 μg/支、2.83 μg/支、1.05 μg/支、1.55 μg/支、0.55 μg/支、2.48 μg/支、6.06 ng/支、3.62 ng/支、0.40 ng/支和6.15 ng/支。
所提出的方法适用于分析卷烟主流烟气中的烟草次要生物碱和烟草特有亚硝胺。
