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黄土高原不同植被类型下的土壤细菌群落

[Soil Bacterial Communities Under Different Vegetation Types in the Loess Plateau].

作者信息

Liu Yang, Huang Yi-Mei, Zeng Quan-Chao

机构信息

Key Laboratory of Plant Nutrition and the Agri-environment of Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China.

出版信息

Huan Jing Ke Xue. 2016 Oct 8;37(10):3931-3938. doi: 10.13227/j.hjkx.2016.10.035.

Abstract

In this study, we collected soil samples from four different arborcommunities and four herb communities, which represented two vegetation ecosystems in the northwest of the Loess Plateau. Our objectives were to determine the diversity of soil bacterial communities and the affecting factors with the method of 454 high-throughput pyrosequencing technology. The results showed that the structures of the microbial communities differed in terms of both the predominant phylum and the relative abundance of each phylum. At the phylum level, the dominant phyla were Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi and Planctomycetes. At the class level, Actinobacteria, -Proteobacteria, Acidobacteria, -Proteobacteria and Planctomycetacia were predominant. The relative abundance of Actinobacteria in grass vegetation ecosystem was more abundant than that in forest vegetation ecosystem. Proteobacteria in forest vegetation ecosystem were more abundant. Soil pH was significantly correlated with the relative abundance of Actinobacteria. Soil organic matter,soil total N and soil total P were the key factors affecting soil Proteobacteria. These results will provide useful information for the diversity of soil bacteria and guide the practice of vegetation restoration in the Loess Plateau.

摘要

在本研究中,我们从四个不同的乔木群落和四个草本群落采集了土壤样本,这些群落代表了黄土高原西北部的两种植被生态系统。我们的目标是采用454高通量焦磷酸测序技术确定土壤细菌群落的多样性及其影响因素。结果表明,微生物群落结构在主要门类和各门类的相对丰度方面均存在差异。在门水平上,优势门类为变形菌门、放线菌门、酸杆菌门、绿弯菌门和浮霉菌门。在纲水平上,放线菌纲、α-变形菌纲、酸杆菌纲、β-变形菌纲和浮霉菌纲占主导地位。草本植被生态系统中放线菌的相对丰度高于森林植被生态系统。森林植被生态系统中变形菌门更为丰富。土壤pH与放线菌的相对丰度显著相关。土壤有机质、土壤全氮和土壤全磷是影响土壤变形菌门的关键因素。这些结果将为土壤细菌多样性提供有用信息,并指导黄土高原植被恢复实践。

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