Liu Xiao-Hua, Wei Tian-Xing
Key Laboratory of State Forestry Administration on Soil and Water Conservation, Engineering Research Center of Forestry Ecological Engineering of Ministry of Education, Jixian County Station, Chinese National Ecosystem Research Network(CNERN), School of Soil and Water Conservation, Beijing Forestry University, Beijing 100083, China.
Huan Jing Ke Xue. 2021 Sep 8;42(9):4489-4499. doi: 10.13227/j.hjkx.202012233.
This study analyzed the characteristics of the soil bacterial community under different types of vegetation restoration, and explored the role of microorganisms in the vegetation restoration process, within the soil ecosystem of Grain for Green areas of the Loess Plateau. Typical artificial forest (artificial forest, forest), natural secondary forest, and grassland were selected as the research objects. High-throughput sequencing technology was used to analyze the diversity and community structure of soil bacteria, and the correlation between soil environmental factors and bacterial communities. The Chinese National Ecosystem Research Network in Jixian County Station was chosen as the research area. The results showed that the bacterial communities of the five types of forest restoration comprised 25 phyla, 66 classes, 129 orders, 240 families, 392 genera, 760 species, and 2213 OTUs. There were significant differences in the abundance of bacterial communities, which could be ranked as follows: CD > CSL > CH2 > CH1 > YS. The dominant bacteria phyla in the different vegetation restoration types were Actinobacteria, Proteobacteria, Acidobacteria, and Chloroflexi. The relative abundances of natural secondary forest, grassland, and plantation were different, but not significantly. The relative abundances of -Proteobacteria, -Proteobacteria, and -Proteobacteria were higher in natural secondary forest soilthan in the soil of the other four restoration models, and the relative abundance was the lowest in grassland soil. The dominant bacteria were significantly correlated with soil pH and TN. Among them, TN was significantly positively correlated with Actinobacteria, pH was significantly positively correlated with Proteobacteria and negatively correlated with Actinobacteria, while Acidobacteria was significantly negatively correlated with soil pH and TN. PICRUSt analysis showed that the functional gene families were mainly related to 24 sub-functions, including cell motility, signal transduction mechanisms, amino acid transport and metabolism, as well as coenzyme transport and metabolism. These results can provide a good foundation for the evaluation of vegetation restoration in the Loess Plateau, as well as a scientific basis for the rational management of plantations.
本研究分析了黄土高原退耕还林地区土壤生态系统中不同植被恢复类型下土壤细菌群落的特征，并探讨了微生物在植被恢复过程中的作用。选取典型人工林（人工林、森林）、天然次生林和草地作为研究对象。采用高通量测序技术分析土壤细菌的多样性和群落结构，以及土壤环境因子与细菌群落之间的相关性。选择中国国家生态系统研究网络蓟县站作为研究区域。结果表明，五种森林恢复类型的细菌群落包括25个门、66个纲、129个目、240个科、392个属、760个种和2213个操作分类单元。细菌群落丰度存在显著差异，排序如下：CD > CSL > CH2 > CH1 > YS。不同植被恢复类型中的优势细菌门为放线菌门、变形菌门、酸杆菌门和绿弯菌门。天然次生林、草地和人工林的相对丰度不同，但差异不显著。天然次生林土壤中α-变形菌纲、β-变形菌纲和γ-变形菌纲的相对丰度高于其他四种恢复模式的土壤，草地土壤中的相对丰度最低。优势细菌与土壤pH值和总氮显著相关。其中，总氮与放线菌门显著正相关，pH值与变形菌门显著正相关，与放线菌门显著负相关，而酸杆菌门与土壤pH值和总氮显著负相关。PICRUSt分析表明，功能基因家族主要与24种亚功能相关，包括细胞运动性、信号转导机制、氨基酸转运和代谢以及辅酶转运和代谢。这些结果可为黄土高原植被恢复评价提供良好基础，也可为人工林的合理经营提供科学依据。
