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基于高通量测序的厌氧折流板自养脱氮反应器各隔室微生物群落特征

[Characteristics of Microbial Community in Each Compartment of ABR ANAMMOX Reactor Based on High-throughput Sequencing].

作者信息

Chen Chong-Jun, Zhang Hai-Qin, Wang Yao-Qi, Yu Xu-Liang, Wang Jian-Fang, Shen Yao-Liang

机构信息

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.

Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou 215009, China.

出版信息

Huan Jing Ke Xue. 2016 Jul 8;37(7):2652-2658. doi: 10.13227/j.hjkx.2016.07.031.

Abstract

In order to investigate the characteristics of microbial community in each compartment of ABR anammox reactor, a five-compartment ABR reactor was used to analyze the microbial community by Miseq High-throughput Sequencing during the steady operational process. The results indicated that the denitrifying bacteria coexisted in the reactor, such as Proteobacteria, Planctomycete, and Nitrospirae bacteria, and the percentages of these three microbial populations in the sludge were 11.66%-20.28%, 2.18%-7.94% and 0.19%-6.30%, respectively. In addition, there were four dominant genera in the phylum Proteobacteria:, , and Furthermore, and were the main genera in the phylum Planctomycete. The color of sludge in the five compartments, in turn, varied from red to black. In addition, the biodiversity index of Chao, ACE, Shannon and Simpson indicated that the richness and diversity of microbial community increased gradually, and at the same time, the relative abundance of Proteobacteria increased while that of Planctomycetes gradually decreased. The above conclusion was consistent with the laws of substrate degradation and enrichment of functional microorganisms.

摘要

为了研究厌氧折流板厌氧氨氧化(ABR)反应器各隔室中微生物群落的特征,采用一个五隔室ABR反应器,在稳定运行过程中通过Miseq高通量测序分析微生物群落。结果表明,反硝化细菌在反应器中共存,如变形菌门、浮霉菌门和硝化螺旋菌门细菌,这三类微生物种群在污泥中的占比分别为11.66%-20.28%、2.18%-7.94%和0.19%-6.30%。此外,变形菌门中有四个优势属: 、 、 和 。此外, 和 是浮霉菌门中的主要属。五个隔室中污泥的颜色依次从红色变为黑色。此外,Chao、ACE、Shannon和Simpson生物多样性指数表明,微生物群落的丰富度和多样性逐渐增加,同时,变形菌门的相对丰度增加,而浮霉菌门的相对丰度逐渐降低。上述结论与底物降解和功能微生物富集规律一致。

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