Yan Bing, Xia Song, Gui Shuang-Lin, Fu Jia-Qi, Wu Jiu-Jiu, Xiong Ji-Hai, Wei Yuan-Song
Institute of Energy, Jiangxi Academy of Sciences, Nanchang 330096, China.
Laboratory of Water Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Huan Jing Ke Xue. 2020 Dec 8;41(12):5535-5543. doi: 10.13227/j.hjkx.202005278.
To understand the changes in microbial community characteristics during the enrichment of anaerobic ammonium oxidation (ANAMMOX) bacteria, an ASBR reactor was used to culture the ANAMMOX bacteria. The composition, diversity, and species co-occurrence network of the microbial community were investigated under different cultivation times. The results showed that the ANAMMOX bacteria were enriched by gradually increasing the substrate concentration, with removal efficiencies for NH-N, NO-N, and total nitrogen of 97.6%, 95.4%, and 84.9%, respectively. The high-throughput sequencing found that the dominant phyla (relative abundance>5%) were Proteobacteria, Bacteroidetes, Chloroflexi, Planctomycetes, Armatimonadetes, and Actinobacteria in the whole culture process. was the main ANAMMOX bacteria in the reactor, with its relative abundance increasing from 1.42% to 24.66%. During the cultivation process, the composition of the dominant microbial community did not change, while the relative abundance showed a significant difference (<0.05). The alpha diversity of the microbial community significantly increased first and then decreased (<0.05), and the beta diversity of the microbial community was significantly spatially differentiated (=0.5672, <0.01) during the culture process. Species network densities were 0.188, 0.068, 0.059, 0.18, and 0.0735 at different times during the culture process. Although the enrichment culture process resulted in weaker correlations between microorganisms, the related group of microorganisms in the phylum Aspergillus became the main node in the network. The enrichment process weakened the correlation between microorganisms; however, the microbial taxa related to the phylum Planctomycetes became the key node in the network.
为了解厌氧氨氧化（ANAMMOX）细菌富集过程中微生物群落特征的变化，采用厌氧序批式反应器（ASBR）培养ANAMMOX细菌。研究了不同培养时间下微生物群落的组成、多样性和物种共现网络。结果表明，通过逐渐提高底物浓度富集了ANAMMOX细菌，NH-N、NO-N和总氮的去除效率分别为97.6%、95.4%和84.9%。高通量测序发现，在整个培养过程中，优势菌门（相对丰度>5%）为变形菌门、拟杆菌门、绿弯菌门、浮霉菌门、装甲菌门和放线菌门。 是反应器中的主要ANAMMOX细菌，其相对丰度从1.42%增加到24.66%。在培养过程中，优势微生物群落的组成没有变化，而相对丰度存在显著差异（<0.05）。微生物群落的α多样性先显著增加后降低（<0.05），培养过程中微生物群落的β多样性在空间上存在显著差异（=0.5672，<0.01）。培养过程中不同时间的物种网络密度分别为0.188、0.068、0.059、0.18和0.0735。虽然富集培养过程导致微生物之间的相关性减弱，但曲霉属相关的微生物群成为网络中的主要节点。富集过程削弱了微生物之间的相关性；然而，与浮霉菌门相关的微生物分类群成为网络中的关键节点。
