Cao Yan, Wang Tong-Yu, Qin Yu-Jie, Han Bin, Ren Jun-Yi
College of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou 510006, China.
Huan Jing Ke Xue. 2017 Apr 8;38(4):1544-1550. doi: 10.13227/j.hjkx.201610081.
The volume loading of upflow blanket filter increased from 0.52 kg·(m·d)to 2.75 kg·(m·d) by improving the influent NH-N and NO-N concentrations. The removal efficiency of NH-N, NO-N and TN increased from 76.18%, 53.47%, 55.66% to 94.04%, 86.97%, 82.96% respectively. Meanwhile, the microbial community of UBF ANAMMOX reactor was analyzed by Illumina high-throughput sequencing during the initial and later operational process. The results indicated that the denitrifying bacteria coexisted in the reactor. The percentages of Planctomycetes, Proteobacteria and Nitrospirae in the sludge were 27.9%-39.9%, 1.1%-26.4% and 0.035%-0.188%, respectively. The proportions of Planctomycetes and Proteobacteria increased from 1.1%, 27.9% in the enrichment stage to 26.4%, 39.9% in the stable operation stage respectively. In addition, Planctomycetes increased significantly. The proportion of Brocadiacea reached 24.57%. Therefore, Brocadiacea became the dominant bacteria in the phylum Planctomycete, which contained . The alpha diversity index and relative abundance of clustering figure indicated that the diversity of microbial community decreased gradually and the structure of microbial community changed obviously.
通过提高进水氨氮和亚硝酸盐氮浓度,上流式厌氧生物滤池的容积负荷从0.52 kg·(m·d)提高到2.75 kg·(m·d)。氨氮、亚硝酸盐氮和总氮的去除效率分别从76.18%、53.47%、55.66%提高到94.04%、86.97%、82.96%。同时,采用Illumina高通量测序技术对UBF厌氧氨氧化反应器运行初期和后期的微生物群落进行了分析。结果表明,反硝化细菌在反应器中共存。污泥中浮霉菌门、变形菌门和硝化螺旋菌门的比例分别为27.9%-39.9%、1.1%-26.4%和0.035%-0.188%。浮霉菌门和变形菌门的比例分别从富集阶段的1.1%、27.9%增加到稳定运行阶段的26.4%、39.9%。此外,浮霉菌门显著增加,其中红杆菌科的比例达到24.57%。因此,红杆菌科成为浮霉菌门中的优势菌属。α多样性指数和聚类图相对丰度表明,微生物群落多样性逐渐降低,群落结构发生明显变化。