Wei Xin, Xue Shun-Li, Yang Fan, Li Xiang, Liu Zhen-Hong, Xue Gang, Gao Pin
State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China.
Huan Jing Ke Xue. 2017 Feb 8;38(2):697-702. doi: 10.13227/j.hjkx.201607235.
To investigate the effects of zero valent iron (Fe) on the decline of antibiotic resistance genes during thermophilic anaerobic digestion of sludge, the abundances of seven tetracycline resistance genes (TC-ARGs, including , and ) and class 1 integron gene () were quantified by quantitative PCR (qPCR). Also, the concentrations of volatile fatty acids (VFAs) were determined. The correlations between the abundances of TC-ARGs and gene and the concentrations of VFAs were discussed. The results showed that appropriate dose of Fe such as 0.10 g·g VSS could enhance the anaerobic digestion process of sludge, and the production of total VFAs and acetic acid increased significantly. The decrease in the abundances of TC-ARGs and gene was also enhanced. However, excessive Fe such as 1.17 g·g VSS could not further improve the reduction in the abundances of TC-ARGs and gene, probably resulted from the occurrence of horizontal gene transfer. The abundances of TC-ARGs except gene, as well as gene exhibited significant negative correlation with the concentration of acetic acid, indicating that acetic acid probably had an enhanced effect on the decline of TC-ARGs and gene during thermophilic anaerobic digestion of sludge.
为研究零价铁（Fe）对污泥嗜热厌氧消化过程中抗生素抗性基因衰减的影响，通过定量聚合酶链反应（qPCR）对7种四环素抗性基因（TC-ARGs，包括 、 和 ）及1类整合子基因（ ）的丰度进行了定量分析。同时，测定了挥发性脂肪酸（VFAs）的浓度。探讨了TC-ARGs和 基因丰度与VFAs浓度之间的相关性。结果表明，适量的Fe（如0.10 g·g VSS）可促进污泥的厌氧消化过程，总VFAs和乙酸的产量显著增加。TC-ARGs和 基因丰度的下降也得到增强。然而，过量的Fe（如1.17 g·g VSS）无法进一步提高TC-ARGs和 基因丰度的降低，这可能是由于水平基因转移的发生。除 基因外的TC-ARGs丰度以及 基因与乙酸浓度呈显著负相关，表明乙酸可能对污泥嗜热厌氧消化过程中TC-ARGs和 基因的衰减具有促进作用。
