Kang Peng-Liang, Huang Ting-Lin, Zhang Hai-Han, Chen Sheng-Nan, Shang Pan-Lu, Feng Ji, Jia Jing-Yu
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Huan Jing Ke Xue. 2017 Dec 8;38(12):5174-5183. doi: 10.13227/j.hjkx.201703179.
Six micro-polluted landscape water bodies were selected to explore the relationship between water quality and the -Type denitrifier community structure and diversity with an Illumina high-throughput sequencing technique. The results of a physico-chemical analysis of the water bodies showed that the Fengqing Park (FQ) and Laodong Park (LD) water bodies were classified as inferior Ⅴ water quality, the Qujiang Park (QJ), Mutasi Park (MTS), and Xinjiyuan Park (XJY) water bodies were classified as Ⅴ water quality, and the Yongyang Park (YY) water quality was classified as Ⅳ. The TN values varied from 1.21 mg·L to 6.50 mg·L, with the lowest value found in YY and the highest in FQ (<0.05). TP was significantly higher in LD (0.10 mg·L) and significantly lower in QJ[0.02 mg·L (<0.05)]. The NH-N in LD was 4.44 times higher than that in QJ and FQ (<0.01). Illumina high-throughput sequencing revealed that the denitrifier community composition was significantly different among the six water bodies, and sp., sp., and sp. were the dominant genus species. A principle component analysis (PCA) indicated that the -Type denitrifier communities of XJY and MTS were mainly regulated by NH-N and the permanganate index, LD and FQ were significantly influenced by NO-N, NO-N, TN, TP, and DO, and YY was significantly influenced by pH value. Our results showed that -Type denitrifier communities were regulated by different water quality indicators.
选取了6个微污染景观水体,采用Illumina高通量测序技术探究水质与反硝化细菌群落结构和多样性之间的关系。水体理化分析结果表明,凤庆公园(FQ)和劳动公园(LD)水体属于劣Ⅴ类水质,曲江公园(QJ)、木塔寺公园(MTS)和辛家庙公园(XJY)水体属于Ⅴ类水质,永阳公园(YY)水质属于Ⅳ类。TN值在1.21 mg·L至6.50 mg·L之间变化,其中YY水体中TN值最低,FQ水体中最高(<0.05)。LD水体中的TP显著高于其他水体(0.10 mg·L),QJ水体中的TP显著低于其他水体[0.02 mg·L(<0.05)]。LD水体中的NH-N比QJ和FQ水体中的NH-N高4.44倍(<0.01)。Illumina高通量测序结果显示,6个水体中反硝化细菌群落组成存在显著差异, sp.、 sp.和 sp.为优势属种。主成分分析(PCA)表明,XJY和MTS的反硝化细菌群落主要受NH-N和高锰酸盐指数调控,LD和FQ受NO-N、NO-N、TN、TP和DO显著影响,YY受pH值显著影响。结果表明,反硝化细菌群落受不同水质指标的调控。