Yang Hao, Zhang Guo-Zhen, Yang Xiao-Ni, Wu Fu-Ping, Zhao Wei, Zhang Hong-Wei, Zhang Xiang
School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.
Engineering Research Center for Cold and Arid Regions Water Resource Comprehensive Utilization, Ministry of Education, Lanzhou 730070, China.
Huan Jing Ke Xue. 2017 Apr 8;38(4):1704-1716. doi: 10.13227/j.hjkx.201608097.
This study was aimed to explore the bacterial diversity of cellar water as well as to study the relationship between the bacterial diversity and environmental factors. The MiSeq high-throughput sequencing was used to analyze and compare the bacterial diversity and community composition of samples from different cellar water samples. Overall 1605 optimized reads were obtained from four samples based on high-throughput sequencing of the V4 region of the 16S rRNA gene. Bacterial species detected in these samples covered 22 phyla,42 classes,71 orders,115 families, 146 genera. Analysis showed that the bacterial diversity was very high in these samples, and there were differences among different samples. The distribution characteristics of the dominant bacteria showed patterns of a large number of rare species and a few common types. Taxonomic assignment analysis indicated that Bacteroidetes,Proteobacteria,Actinobacteria,Verrucomicrobia,OD1 dominated in the Cellar water, and accounted for 87.1% to 94.8% at phylum level. The predominant groups were Actinobacteria,Acidimicrobiia,Cytophagia, Flavobacteriia, Sphingobacteriia,-Proteobacteria,-Proteobacteria,-Proteobacteria,Opitutae, Verrucomicrobiae,Pedosphaerae and ZB2 at class level. At genus level were the abundant taxa, which were mainly denitrifying bacteria and heterotrophic nitrification-aerobic denitrification bacteria. The result of RDA suggested that the influences of different environmental factors on different microbes were different. Bacterial community Ⅱ had significant positive correlation with UV,permanganate index,BOD,and Bacterial community Ⅲ had significant positive correlation with TN,NO-N,NO-N,TP,NH-N. This research should deepen the understanding on microbial community in Cellar water, and provide references for the association of bacterial composition and diversity with environmental factors.
本研究旨在探究窖藏水的细菌多样性，并研究细菌多样性与环境因素之间的关系。采用MiSeq高通量测序技术对不同窖藏水样本的细菌多样性和群落组成进行分析和比较。基于16S rRNA基因V4区的高通量测序，从4个样本中总共获得了1605条优化后的 reads。这些样本中检测到的细菌种类涵盖22个门、42个纲、71个目、115个科、146个属。分析表明，这些样本中的细菌多样性非常高，且不同样本之间存在差异。优势菌的分布特征呈现出大量稀有物种和少数常见类型的模式。分类学归属分析表明，拟杆菌门、变形菌门、放线菌门、疣微菌门、OD1在窖藏水中占主导地位，在门水平上占87.1%至94.8%。在纲水平上，主要优势类群为放线菌纲、酸微菌亚纲、噬细胞菌纲、黄杆菌纲、鞘脂杆菌纲、α-变形菌纲、β-变形菌纲、γ-变形菌纲、疣微菌纲、OP11、Pedosphaerae和ZB2。在属水平上有丰富的分类单元，主要是反硝化细菌和异养硝化-好氧反硝化细菌。冗余分析结果表明，不同环境因素对不同微生物的影响不同。细菌群落Ⅱ与紫外线、高锰酸盐指数、生化需氧量呈显著正相关，细菌群落Ⅲ与总氮、亚硝酸盐氮、硝酸盐氮、总磷、氨氮呈显著正相关。本研究有助于加深对窖藏水中微生物群落的理解，并为细菌组成和多样性与环境因素的关联提供参考。
