Zhang Lan-He, Wang Jia-Jia, Ha Xue-Jiao, Qiu Meng-Chao, Gao Min, Qiu Tian-Lei, Wang Xu-Ming
College of Chemical Engineering, Northeast Dianli University, Jilin 132012, China.
Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Huan Jing Ke Xue. 2016 Nov 8;37(11):4395-4401. doi: 10.13227/j.hjkx.201605077.
In order to study the distribution characteristics and pollution status of antibiotic resistance genes (ARGs) in the vegetable soils, greenhouse and field soil samples in 11 organic vegetable bases with long-term manure amended in Beijing were collected. All samples were subjected to the determination of 13-type antibiotics and related ARGs. The experimental results showed that tetracycline residues were the highest among all antibiotics tested, followed by sulfanilamides. Antibiotic residues were higher in greenhouse soils than in field soils. For all soil samples, sulfanilamide resistance genes and , as well as the tetracycline resistance gene were observed with 100% detection frequencies. Detection frequency for class I integron () in greenhouse soils was 1.5 times higher than that in field soils. The relative abundance for and in soil samples ranged 10-10 as found by quantitative PCR (qPCR) detection. The relative abundance of was significantly positively correlated with the contents of sulfadimidine and doxycycline (<0.05), and the relative abundance of did not exhibit evident correlation with the contents of antibiotics tested. These results would contribute to understanding of the pollution status of ARGs in vegetable soils in Beijing, and to evaluation of currently agricultural practices based on ARGs.
为研究北京11个长期施用有机肥的有机蔬菜基地菜地土壤、温室土壤和大田土壤中抗生素抗性基因（ARGs）的分布特征及污染状况，采集了上述土壤样品。对所有样品进行了13种类型抗生素及相关ARGs的测定。实验结果表明，在所检测的所有抗生素中，四环素残留量最高，其次是磺胺类药物。温室土壤中的抗生素残留量高于大田土壤。对于所有土壤样品，磺胺类抗性基因和，以及四环素抗性基因的检出频率均为100%。温室土壤中I类整合子（）的检出频率比大田土壤高1.5倍。通过定量PCR（qPCR）检测发现，土壤样品中和的相对丰度范围为10-10。的相对丰度与磺胺嘧啶和强力霉素的含量呈显著正相关（<0.05），而的相对丰度与所检测抗生素的含量未表现出明显相关性。这些结果将有助于了解北京菜地土壤中ARGs的污染状况，并基于ARGs对当前农业实践进行评估。
