[梅花鹿养殖场抗生素抗性基因的分布特征]
[Distribution Characteristics of Antibiotic Resistance Genes in Sika Deer Farm].
作者信息
Huang Fu-Yi, An Xin-Li, Chen Qing-Lin, Ren Hong-Yun, Su Jian-Qiang
机构信息
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
出版信息
Huan Jing Ke Xue. 2016 Nov 8;37(11):4402-4409. doi: 10.13227/j.hjkx.201605196.
Sika deer breeding is rapidly developing in South China during the recent years; however, the impact of sika deer breeding on the abundance and diversity of antibiotic resistance genes (ARGs) in sika deer farm remains elusive. In this study, profiles of ARGs were investigated using high-throughput qPCR technique. The results showed that the abundance of ARGs varied among different samples in the following order, deer manure composting > deer manure > vegetable soil > pristine soil, and the profiles of ARGs in these samples were significantly different (<0.05). The abundance of ARGs was significantly correlated to that of mobile genetic elements (MGEs), implicating the potential of horizontal transfer of ARGs, which might accelerate the dissemination and enrichment of ARGs in the environment of sika deer farm.
近年来，梅花鹿养殖在中国南方迅速发展；然而，梅花鹿养殖对梅花鹿养殖场抗生素抗性基因（ARGs）丰度和多样性的影响仍不清楚。在本研究中，使用高通量qPCR技术研究了ARGs的谱图。结果表明，不同样品中ARGs的丰度按以下顺序变化：鹿粪堆肥>鹿粪>蔬菜土壤>原始土壤，且这些样品中ARGs的谱图有显著差异（<0.05）。ARGs的丰度与移动遗传元件（MGEs）的丰度显著相关，这意味着ARGs存在水平转移的可能性，这可能会加速ARGs在梅花鹿养殖场环境中的传播和富集。
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