Gu Shu-Jun, Fang Fang, Li Kai, Liu Yong, Guo Jin-Song, Chen You-Peng, Jiang Fu-Yang
College of Urban Construction & Environmental Engineering, Chongqing University, Chongqing 400045, China.
Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 401122, China.
Huan Jing Ke Xue. 2016 Aug 8;37(8):3120-3127. doi: 10.13277/j.hjkx.2016.08.038.
Biofilm coming from a reactor in which One-stage Autotrophic Nitrogen Removal Process exists was selected as inoculum in an expanded granular sludge bed reactor. A potential fast start-up procedure was tested in this research. Wastewater with low ammonium concentration between 60-100 mg·L was applied. The results showed that a One-stage Autotrophic Nitrogen Removal Process was successfully established in 83 days under the following conditions: temperature at (30±2)℃ , pH at 7.8-8.2, dissolved oxygen (DO) at 0.2-1.1mg·L and upflow velocity at 2.0-4.0m·h. After the 83-day operation, the removal efficiencies of NH-N and TN were 99.4% and 80.7%, respectively. By controlling the reflux ratio and increasing the NH-N load, the reactor could maintain a stable state of low DO concentration. Nitrification and anaerobic ammonium oxidation became main reactions that maintained efficient and stable nitrogen removal performance. In addition, nitrite-oxidizing bacteria (NOB) were inhibited. In the start-up process, the average particle diameter of granular sludge increased from 174 to 296 μm. Moreover, scanning electron microscopy showed that the surface of granular sludge was smooth, and most microorganisms were bevibacteria and cocci. Finally, a fluorescence in situ hybridization experiment showed that ammonium oxidation bacteria and anaerobic ammonium oxidation bacteria were distributed on the surface and in the inner space of granular sludge, respectively. The research indicated that a stable autotrophic nitrogen removal granular sludge system was quickly established in the sludge bed.
选取来自存在单级自养脱氮工艺的反应器中的生物膜作为膨胀颗粒污泥床反应器的接种物。本研究测试了一种潜在的快速启动程序。采用了铵浓度在60 - 100mg·L之间的低浓度废水。结果表明，在温度为(30±2)℃、pH为7.8 - 8.2、溶解氧(DO)为0.2 - 1.1mg·L、上流速度为2.0 - 4.0m·h的条件下，83天内成功建立了单级自养脱氮工艺。运行83天后，NH-N和TN的去除效率分别为99.4%和80.7%。通过控制回流比和增加NH-N负荷，反应器能够维持低溶解氧浓度的稳定状态。硝化和厌氧氨氧化成为维持高效稳定脱氮性能的主要反应。此外，亚硝酸氧化菌(NOB)受到抑制。在启动过程中，颗粒污泥的平均粒径从174μm增加到296μm。而且，扫描电子显微镜显示颗粒污泥表面光滑，大多数微生物为短杆菌和球菌。最后，荧光原位杂交实验表明，氨氧化菌和厌氧氨氧化菌分别分布在颗粒污泥的表面和内部空间。该研究表明在污泥床中快速建立了稳定的自养脱氮颗粒污泥系统。
