Xu Ping-Ping, Liu Cong, Wang Ya, Zheng Yan-Heng, Zhang Chun-Hua, Ge Ying
Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Laboratory Centre of Life Science, Demonstration Laboratory of Elements and Life Science, Nanjing Agricultural University, Nanjing 210095, China.
Huan Jing Ke Xue. 2016 Sep 8;37(9):3438-3446. doi: 10.13227/j.hjkx.2016.09.023.
Algae-bacteria consortia may be potentially applied in wastewater treatment and environment remediation. In this study, in order to investigate effects of a symbiotic bacterium on the accumulation and transformation of arsenate[As(Ⅴ)] by , we used batch cultures to determine the uptake, adsorption and transformation of As by axenic and non-axenic exposed to 0-750 μg·L As(Ⅴ) for 7 d. The symbiotic bacterium of was confirmed to be sp. after isolation, cultivation and 16S rRNA identification. The bacterial presence markedly increased the adsorption of As in , but it markedly reduced the absorption and the toxic effect of As(Ⅴ). Arsenate was the major arsenic species in the cells of axenic and non-axenic . The proportion of arsenite[As(Ⅲ)] was 8.99%-11.52% in the axenic microalga whereas a small quantity of monomethylarsonous acid (MMA) and dimethylarsinous acid (DMA) (0.02%-0.04%) were detected in the non-axenic counterpart. As(Ⅲ) dominated the As speciation in the bacterial culture and the percentage of As(Ⅴ) was 7.59%-26.80%, indicating that this symbiotic bacterium had a strong As(Ⅴ) reducing ability. The As removal rate (19.81%-41.08%) by non-axenic was higher than the bacterium alone (5.14%-14.62%) and axenic (14.98%-21.08%) after 7 d As(Ⅴ) exposure. The symbiotic sp. promoted the accumulation of As by , indicating that algae-bacteria consortia might enhance the bioremediation of As contaminated water.
藻菌共生体可能在废水处理和环境修复中具有潜在应用价值。在本研究中，为了探究一种共生细菌对砷酸盐[As(Ⅴ)]在[具体藻类名称未给出]中的积累和转化的影响，我们采用分批培养法来测定无菌和有菌的[具体藻类名称未给出]在暴露于0 - 750 μg·L As(Ⅴ) 7天的情况下对砷的吸收、吸附和转化情况。经分离、培养和16S rRNA鉴定后，确定[具体藻类名称未给出]的共生细菌为[具体细菌名称未给出]菌。细菌的存在显著增加了[具体藻类名称未给出]对砷的吸附，但显著降低了对As(Ⅴ)的吸收及其毒性作用。砷酸盐是无菌和有菌的[具体藻类名称未给出]细胞中的主要砷形态。在无菌微藻中，亚砷酸盐[As(Ⅲ)]的比例为8.99% - 11.52%，而在有菌的微藻中检测到少量的一甲基亚砷酸（MMA）和二甲基亚砷酸（DMA）（0.02% - 0.04%）。As(Ⅲ)在细菌培养物中的砷形态中占主导地位，As(Ⅴ)的百分比为7.59% - 26.80%，表明这种共生细菌具有很强的As(Ⅴ)还原能力。在暴露于As(Ⅴ) 7天后，有菌的[具体藻类名称未给出]对砷的去除率（19.81% - 41.08%）高于单独的细菌（5.14% - 14.62%）和无菌的[具体藻类名称未给出]（14.98% - 21.08%）。共生的[具体细菌名称未给出]菌促进了[具体藻类名称未给出]对砷的积累，表明藻菌共生体可能增强对砷污染水体的生物修复作用。
