Yu Qing-Nan, Chen Shuang-Shuang, Zheng Chao, Zhang Chun-Hua, Ge Ying
Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Laboratory Centre of Life Science, Demonstration Laboratory of Elements and Life Science, Nanjing Agricultural University, Nanjing 210095, China.
Ying Yong Sheng Tai Xue Bao. 2020 Oct;31(10):3539-3546. doi: 10.13287/j.1001-9332.202010.032.
We constructed consortia of and with various alga-bacterium ratios (1:0, 1:1, 1:2, 1:3, 1:4). After being treated with arsenate [As(Ⅴ)] for 7 d, we measured the growth, As accumulation, adsorption and absorption, and As speciation transformation of consortia. Results showed that the chlorophyll content, dry weight, and specific growth rate of the symbiont increased significantly with increasing ratio after the As(Ⅴ) treatment, being 1.81 mg·L, 125.0 mg, and 0.28 mg·L·d under the condition of the alga-bacterium ratio being 1∶4 and As(Ⅴ) being 750 μg·L, respectively. The accumulation and absorption of As by the consortia decreased with the bacterial proportion increasing from 1:0 to 1:4. As accumulation changed with the As concentration, with a dominance of absorption under 75-150 μg·L As(Ⅴ) and a dominance of adsorption under 300-750 μg·LAs(V). There were As(Ⅴ) and As(Ⅲ) in the consortia. When the proportion of bacteria increased, the rate of As(Ⅴ) reduction enhanced (up to 12.6%). Our results indicated that the increases of improved As(Ⅴ) tolerance and reduction, but decreased the As(Ⅴ) accumulation by the symbiont.
