Sun Guang-Xi, Jin Peng-Kang, Song Ji-Na, Wang Xian-Bao, Yang Ke-Yao
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Huan Jing Ke Xue. 2016 Jun 8;37(6):2252-2258. doi: 10.13227/j.hjkx.2016.06.031.
A study was conducted through a 1200 m-PVC-pipe-reactor, which was used to simulate the urban sewer system. Gas chromatography, liquid chromatography and 454 high-throughput sequencing were utilized to study the variation of substrates during the methanogenic process and the distribution characteristics of methanogens in the sewer system. The results showed that the concentration of methane increased along the sewer system, which illustrated that methanogens existed in the sewer network. The methanogens mainly contained _unclassified and _unclassified. The distinct succession which _unclassified replaced to be the first dominant microbial genus between 800-1000 m of the sewer system. Formic acid, methanol, methylamine, acetic acid and hydrogen were available substrates for methanogens. Among these substrates, acetic acid was the primary substrate for methanogen. The variation trends of these substrates were first increasing and then decreasing along the length of the sewer system, which led to the succession phenomenon of methanogens in the sewer system.
通过一个1200米的聚氯乙烯管道反应器进行了一项研究，该反应器用于模拟城市下水道系统。利用气相色谱、液相色谱和454高通量测序来研究产甲烷过程中底物的变化以及下水道系统中产甲烷菌的分布特征。结果表明，甲烷浓度沿下水道系统增加，这说明下水道网络中存在产甲烷菌。产甲烷菌主要包含未分类的和未分类的。在下水道系统800 - 1000米之间，未分类的取代成为第一优势微生物属，出现了明显的演替。甲酸、甲醇、甲胺、乙酸和氢气是产甲烷菌的可用底物。在这些底物中，乙酸是产甲烷菌的主要底物。这些底物的变化趋势沿下水道系统长度先增加后减少，这导致了下水道系统中产甲烷菌的演替现象。
