Zhang Qian, Ji Fang-Ying, Xu Xuan
School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China.
Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Huan Jing Ke Xue. 2018 Apr 8;39(4):1763-1772. doi: 10.13227/j.hjkx.201709049.
To solve the problems of high energy consumption, complex process, and low nitrogen removal efficiency of the currently available low carbon source wastewater treatment processes, a novel coagulation sedimentation/post solid-phase denitrification biofilter process was proposed. Domestic wastewater with low carbon to nitrogen ratio was treated. The changes in the microbial community structure along the biofilters were studied and the functional bacteria were identified using the polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that the microbial diversity and richness of the microorganisms increased from the bottom to the top along the nitrification filter, while in the denitrification filter, there parameters increased firstly and then decreased from the bottom to the top along the cylinder. A bigger population of nitrifying and denitrifying bacteria was observed at the top and the middle of the nitrification and denitrification biofilter, respectively. Moreover, sp. Nm47 and Candidatus were the predominant nitrifying bacteria in the nitrification biofilter, and and were the predominant denitrifying bacteria in the solid-phase denitrification biofilter.
为解决现有低碳源污水处理工艺能耗高、流程复杂、脱氮效率低等问题，提出了一种新型的混凝沉淀/后置固相反硝化生物滤池工艺。对低碳氮比的生活污水进行了处理。利用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）研究了沿生物滤池微生物群落结构的变化，并鉴定了功能细菌。结果表明，沿硝化滤池从底部到顶部，微生物的多样性和丰富度增加，而在反硝化滤池中，这些参数沿滤池从底部到顶部先增加后减少。在硝化和反硝化生物滤池的顶部和中部，分别观察到较大数量的硝化细菌和反硝化细菌。此外，Nm47菌属和硝化螺菌属是硝化生物滤池中的主要硝化细菌，而不动杆菌属和假单胞菌属是固相反硝化生物滤池中的主要反硝化细菌。
