Ruggiero M, Zimmerman T P, Lapetina E G
Biochem Biophys Res Commun. 1985 Sep 16;131(2):620-7. doi: 10.1016/0006-291x(85)91282-3.
Saponin (5 to 25 micrograms/ml) produced a concentration-dependent decrease in the cellular content of total ATP and [32P]ATP in 32P-labeled human platelets. In platelets whose ATP had been profoundly decreased by saponin, Ca2+ produced phosphomonoesteratic cleavage of the polyphosphoinositides with a concomitant accumulation of phosphatidylinositol. Collagen still induced secretion of serotonin in platelets that had been treated with saponin in the presence or absence of Ca2+. This effect of collagen occurred in the absence of the formation of cyclooxygenase metabolites. In platelet permeabilized with saponin, agonist-induced secretion and aggregation seems to be unrelated to protein phosphorylation, breakdown of the inositol phospholipids by phospholipase C and formation of cyclooxygenase metabolites.
皂苷（5至25微克/毫升）使32P标记的人血小板中总ATP和[32P]ATP的细胞含量呈浓度依赖性降低。在ATP因皂苷而大幅降低的血小板中，Ca2+导致多磷酸肌醇发生磷酸单酯酶裂解，并伴随磷脂酰肌醇的积累。在有或没有Ca2+存在的情况下，胶原蛋白仍能诱导经皂苷处理的血小板分泌5-羟色胺。胶原蛋白的这种作用在没有环氧化酶代谢产物形成的情况下发生。在用皂苷通透的血小板中，激动剂诱导的分泌和聚集似乎与蛋白质磷酸化、磷脂酶C对肌醇磷脂的分解以及环氧化酶代谢产物的形成无关。
