离子载体A23187可诱导凝血酶激活的肌醇磷酸释放进入不应期状态。
Ionophore A23187 induces a refractory state in thrombin-activated release of inositol phosphates.
作者信息
Moscat G, Moreno F, Iglesias S, Garcia-Barreno P, Municio A M
出版信息
Biochem J. 1986 Sep 15;238(3):709-14. doi: 10.1042/bj2380709.
Abstract
The phosphatidylinositol cycle has been proposed to be involved in the regulation of platelet functionality through the control of cytoplasmic Ca2+ levels. However, the requirements of phospholipase C for Ca2+ has not yet been elucidated in intact platelets. The primary purpose of the present study was to investigate the Ca2+ requirements of this enzyme in platelets from miniature swine by taking advantage of the permeabilizing properties of the ionophore A23187. Our results strongly suggest that the treatment of platelets with A23187 induces a refractory state in thrombin-stimulated release of inositol phosphates while 5-hydroxytryptamine (serotonin)-secretory capacity in response to thrombin remained constant. This refractory state seems to be dependent on some cytochalasin-inhibitable cytoskeletal phenomena.
摘要
磷脂酰肌醇循环被认为通过控制细胞质钙离子水平参与血小板功能的调节。然而，完整血小板中磷脂酶C对钙离子的需求尚未阐明。本研究的主要目的是利用离子载体A23187的通透特性，研究小型猪血小板中该酶对钙离子的需求。我们的结果强烈表明，用A23187处理血小板会在凝血酶刺激的肌醇磷酸释放中诱导一种不应状态，而凝血酶刺激的5-羟色胺（血清素）分泌能力保持不变。这种不应状态似乎依赖于某些细胞松弛素可抑制的细胞骨架现象。
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