Wang Wen-Xiao, Bian Wei, Wang Meng, Kan Rui-Zhe, Zhao Qing, Liang Dong-Bo, Li Jun
College of Architecture and Civil Engineering of Beijing University of Technology, Beijing 100124, China.
Beijing Drainage Group Co., Ltd., Beijing 100044, China.
Huan Jing Ke Xue. 2017 Oct 8;38(10):4332-4339. doi: 10.13227/j.hjkx.201703207.
Four mini experiments were conducted at different conditions. The heterotrophic microorganisms on the aerobic granular sludge surface consumed organic compounds at the initial stage of aeration. The denitrification rate and the efficiency of NO-N and NO-N removal were relatively low. Therefore, under the normal temperature conditions (20-23℃), aerobic granular sludge sequencing batch reactor (SBR) was operated in the two-stage aeration mode(first in low aeration then in high aeration mode). The low aeration time were carried out at 1, 2 and 3 hours stages respectively, and the characteristics of the granular sludge and its effects on microorganisms were analyzed by scanning electron microscopy (SEM) and fluorescence in situ hybridization (FISH) technique. The results show that the increase in the aerobic granular sludge (AGS) particle size improved the denitrification capacity; the denitrification rate of NO-N was the highest at low aeration mode with 2 h and reached 9.66 mg·(g·h). The accumulation rate of nitrite increased to 77.84% and the total nitrogen removal rate to 70%. The bacterial count inside the granular sludge increased and they were mainly cocci, bacillus, and ellipsoidal bacteria. Moreover, the proportion of ammonia-oxidizing bacteria in total bacterial count increased from 13.70% to 15.40%. Therefore, the two-stage aeration process achieved shortened simultaneous nitrification and denitrification processes and showed a good denitrification performance.
在不同条件下进行了四个小型实验。曝气颗粒污泥表面的异养微生物在曝气初期消耗有机化合物。反硝化速率以及NO-N和NO-N的去除效率相对较低。因此，在常温条件（20-23℃）下，曝气颗粒污泥序批式反应器（SBR）采用两阶段曝气模式运行（先低曝气后高曝气模式）。低曝气时间分别在1小时、2小时和3小时阶段进行，并通过扫描电子显微镜（SEM）和荧光原位杂交（FISH）技术分析颗粒污泥的特性及其对微生物的影响。结果表明，曝气颗粒污泥（AGS）粒径的增加提高了反硝化能力；在2小时的低曝气模式下，NO-N的反硝化速率最高，达到9.66 mg·(g·h)。亚硝酸盐积累率增加到77.84%，总氮去除率达到70%。颗粒污泥内部细菌数量增加，主要为球菌、杆菌和椭圆形细菌。此外，氨氧化细菌在总细菌数量中的比例从13.70%增加到15.40%。因此，两阶段曝气过程实现了同步硝化反硝化过程的缩短，并表现出良好的反硝化性能。
