Mu Dong-Yang, Jin Peng-Fei, Peng Yong-Zhen, Li Xi-Yao, Zhang Qiong, He Jian-Zhong
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.
Department of Civil & Environmental Engineering, National University of Singapore, Singapore 117576, Singapore.
Huan Jing Ke Xue. 2017 Nov 8;38(11):4763-4773. doi: 10.13227/j.hjkx.201704131.
Nitrogen removal by a newly discovered sp. strain, DK1, isolated from activated sludge was investigated. Using glucose as a carbon source and a (C)/(N) ratio of five, batch experiments showed that the aerobic denitrification removal rate was 4.09 mg·(L·h) and 4.43 mg·(L·h) with NaNO or NaNO, respectively. Completely nitrogen removal was achieved when using these two nitrogen sources. DK1 was also found to heterotrophically remove NH -N at a rate of 2.32 mg·(L·h) and to carry out anoxic denitrification of a range of concentrations of NO -N (from about 100 to 300 mg·L) within a maximum of 36 hours of inoculation. In the presence of both NO -N and NO -N, DK1 was found to preferentially denitrify NO -N. Simultaneous nitrification and denitrification (SND) capacity of the DK1 strain was observed when using ammonium and nitrate or ammonium and nitrite and the corresponding nitrogen removal rates reached as high as 95.06% and 94.69% within 30 hours of inoculation, respectively. Ammonium with both nitrate and nitrite resulted in a 100% nitrogen removal within the same time frame. The ability to achieve SND and to denitrify both NO -N and NO -N makes the DK1 strain potentially useful for future application in nitrogenous wastewater treatment.
对从活性污泥中分离出的新发现的sp.菌株DK1的脱氮性能进行了研究。以葡萄糖作为碳源，碳氮比为5，批次实验表明，以NaNO₂或NaNO₃为氮源时，好氧反硝化去除率分别为4.09 mg·(L·h)和4.43 mg·(L·h)。使用这两种氮源时均可实现完全脱氮。还发现DK1能够以2.32 mg·(L·h)的速率异养去除NH₄⁺-N，并在接种后最多36小时内对一系列浓度（约100至300 mg·L）的NO₃⁻-N进行缺氧反硝化。在同时存在NO₂⁻-N和NO₃⁻-N的情况下，发现DK1优先对NO₂⁻-N进行反硝化。当使用铵盐与硝酸盐或铵盐与亚硝酸盐时，观察到DK1菌株具有同时硝化反硝化（SND）能力，相应的脱氮率在接种后30小时内分别高达95.06%和94.69%。铵盐与硝酸盐和亚硝酸盐同时存在时，在相同时间内可实现100%脱氮。DK1菌株实现SND以及对NO₂⁻-N和NO₃⁻-N进行反硝化的能力使其在未来含氮废水处理中具有潜在应用价值。
