[Nitrifying Bacteria Culture in Entrapment Immobilization].

In order to realize the industrialization of nitrifying bacteria enrichment cultivation and large-scale application of entrapment immobilization, activated sludge from a sewage treatment plant was used as the seed sludge. The concentration of substrate was increased progressively in an industrialized tank. Nitrifying bacteria were grown rapidly by controlling the concentration of FA and FNA, and the ammonia oxidation rate reached 118 mg·(L·h) eventually. The results of high-throughput sequencing showed that , nitrifying bacteria, was only 0.53% of the bacteria. After exposing it to an enrichment culture, the content of rose to 10.27%, 20 times larger than that in the raw sludge. Diversity was significantly smaller at the same time. After embedding immobilization with PVA (polyvinyl alcohol), the embedded fillers nitrification load was 30%. A continuous-flow approach was utilized in the recovery phase, and the nitrification loading rate reached a high level, 62 mg·(L·h), and efficient recovery of the biologically active fillers was achieved.