Yu Meng-Yu, Liu Yi, Tian Yu-Bin, Shi Huan, Xu Fu, Yang Hong
Key Laboratory for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
Huan Jing Ke Xue. 2017 Jul 8;38(7):2925-2930. doi: 10.13227/j.hjkx.201701089.
In order to improve the efficiency of shortcut nitrification by entrapping ammonia oxidizing bacteria, the technique of ammonia oxidizing bacteria(AOB) enrichment culture was studied. The continuous operation method was used to inhibit the growth of nitrite-oxidizing bacteria(NOB) by free ammonia, at the same time, the NOB was gradually washed out of the system through the technology of sludge discharge. Polyvinyl alcohol(PVA) was used as the embedding material to immobilize the ammonia oxidizing bacteria after enrichment culture. The embedding rate of the reactor was 8%. The ammonia oxidation rate was increased by improving ammonia nitrogen loading in the continuous operation mode. The results showed that the ammonia oxidation rate(NH-N/VSS)was as high as 2.028 g·(g·d) and the nitrite nitrogen accumulation rate was stabilized at more than 90%. High-throughput sequencing analysis was conducted on the bacterial community composition before and after enrichment culture, and the experimental result indicated that the culture diversity of raw sludge was larger. and , which have nitrification function, accounted for 0.24% and 2.7%, respectively. The diversity of the activated sludge decreased significantly after the enrichment culture, (18%) became the dominant bacteria whereas occupied only 0.02%. After he embedding of ammonia oxidizing bacteria, the shortcut nitrification was realized rapidly. Finally, the shortcut nitrification rate reached 50 mg·(L·h)and the nitrite nitrogen accumulation was above 90%.
为了通过截留氨氧化细菌提高短程硝化效率，研究了氨氧化细菌（AOB）富集培养技术。采用连续运行方法，利用游离氨抑制亚硝酸盐氧化细菌（NOB）的生长，同时通过排泥技术将NOB逐渐从系统中洗出。富集培养后的氨氧化细菌采用聚乙烯醇（PVA）作为包埋材料进行固定化。反应器的包埋率为8%。在连续运行模式下，通过提高氨氮负荷来提高氨氧化速率。结果表明，氨氧化速率（NH-N/VSS）高达2.028 g·(g·d)，亚硝酸盐氮积累率稳定在90%以上。对富集培养前后的细菌群落组成进行高通量测序分析，实验结果表明，原污泥的培养多样性较大。具有硝化功能的 和 分别占0.24%和2.7%。富集培养后活性污泥的多样性显著降低，（18%）成为优势菌，而 仅占0.02%。氨氧化细菌包埋后，短程硝化迅速实现。最终，短程硝化速率达到50 mg·(L·h)，亚硝酸盐氮积累率在90%以上。
