Zhang Yu, Mi Tie-Zhu, Zhen Yu, Chen Ye, Fu Lu-Lu, Wang Xun-Gong
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao 266100, China.
Huan Jing Ke Xue. 2018 Jan 8;39(1):438-449. doi: 10.13227/j.hjkx.201705054.
Sulfate-reducing prokaryotes (SRP) and sulfur-oxidizing prokaryotes (SOP) play vital roles in the sulfur cycle. The SRP community was used to represent a microbial community with high richness and diversity. The 454 pyrosequencing, Illumina high-throughput sequencing, and traditional clone library methods that target the dissimilatory sulfite reductase subunit gene (), which encodes a key enzyme in the sulfate reduction pathway, were used to compare the differences in SRP community characteristics. Comparative analyses suggested that Illumina high-throughput sequencing was a more appropriate method for SRP (high richness and diversity) community studies. The SOP gene (~750 bp) was used as a representative of the long-sequence segment. The 454 pyrosequencing and Illumina high-throughput sequencing methods were used to compare the differences in SOP community characteristics. The results revealed that 454 pyrosequencing did not reflect its advantage of a longer read length; whereas, the Illumina high-throughput sequencing with more numerous and shorter sequence reads was more suitable when the gene was used to investigate the community composition and diversity of SOP.
硫酸盐还原原核生物（SRP）和硫氧化原核生物（SOP）在硫循环中发挥着至关重要的作用。SRP群落被用来代表具有高丰富度和多样性的微生物群落。靶向异化亚硫酸盐还原酶亚基基因（ ）（该基因编码硫酸盐还原途径中的一种关键酶）的454焦磷酸测序、Illumina高通量测序和传统克隆文库方法，被用于比较SRP群落特征的差异。比较分析表明，Illumina高通量测序是研究SRP（高丰富度和多样性）群落更为合适的方法。SOP基因（约750 bp）被用作长序列片段的代表。454焦磷酸测序和Illumina高通量测序方法被用于比较SOP群落特征的差异。结果显示，454焦磷酸测序并未体现出其读长较长的优势；而当使用 基因来研究SOP的群落组成和多样性时，具有更多数量且较短序列读段的Illumina高通量测序更为合适。
