深海来源真菌的从头转录组测序和Gliotoxin 生物合成基因分析。

De Novo Transcriptome Sequencing of the Deep-Sea-Derived Fungus and Analysis of Gliotoxin Biosynthesis Genes.

机构信息

State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 100 Central Xianlie Road, Yuexiu District, Guangzhou 510070, China.

出版信息

Int J Mol Sci. 2018 Jun 29;19(7):1910. doi: 10.3390/ijms19071910.

DOI:10.3390/ijms19071910

PMID:29966253

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6073683/

Abstract

Gliotoxin, produced by fungi, is an epipolythiodioxopiperazine (ETP) toxin with bioactivities such as anti-liver fibrosis, antitumor, antifungus, antivirus, antioxidation, and immunoregulation. Recently, cytotoxic gliotoxins were isolated from a deep-sea-derived fungus, . However, the biosynthetic pathway for gliotoxins in remains unclear. In this study, the transcriptome of was sequenced using an Illumina Hiseq 2000. A total of 19,125 unigenes for were obtained from 9.73 GB of clean reads. Ten genes related to gliotoxin biosynthesis were annotated. The expression levels of gliotoxin-related genes were detected through quantitative real-time polymerase chain reaction (qRT-PCR). The gene, encoding a glutathione -transferase (DC-GST); , encoding an aminotransferase (DC-AI); and , encoding an aldehyde reductase (DC-AR), were cloned and expressed, purified, and characterized. The results suggested the important roles of DC-GST, DC-AT, and DC-AR in the biosynthesis of gliotoxins. Our study on the genes related to gliotoxin biosynthesis establishes a molecular foundation for the wider application of gliotoxins from in the biomedical industry in the future.

摘要

真菌产生的Gliotoxin 是一种具有抗肝纤维化、抗肿瘤、抗真菌、抗病毒、抗氧化和免疫调节等生物活性的 Epipolythiodioxopiperazine (ETP) 毒素。最近，从深海来源的真菌中分离出细胞毒性Gliotoxin。然而，中Gliotoxin 的生物合成途径尚不清楚。在本研究中，使用 Illumina Hiseq 2000 对的转录组进行了测序。从 9.73GB 的清洁读取中获得了 19125 个的 unigenes。注释了与 Gliotoxin 生物合成相关的 10 个基因。通过定量实时聚合酶链反应 (qRT-PCR) 检测了Gliotoxin 相关基因的表达水平。克隆并表达、纯化和表征了编码谷胱甘肽 -转移酶 (DC-GST)的基因、编码氨基转移酶 (DC-AI)的基因和编码醛还原酶 (DC-AR)的基因。结果表明，DC-GST、DC-AT 和 DC-AR 在 Gliotoxin 的生物合成中起重要作用。我们对Gliotoxin 生物合成相关基因的研究为未来更广泛地将中的 Gliotoxin 应用于生物医学工业奠定了分子基础。