Borukhov S I, Izotova L S, Kostrov S V, Staroverov S M, Musatova A A
Biokhimiia. 1985 Sep;50(9):1471-80.
Chromatography on immobilized monoclonal antibodies NK-2 from a bacterial strain-producer resulted in a pure human leukocyte alpha-interferon A (alpha-INF-A) homogeneous upon polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and reverse phase high performance liquid chromatography. The chromatographic properties of partially purified alpha-INF-A on synthetic and commercial sorbents containing immobilized dyes, aromatic dipeptides, chelating and hydrophobic ligands as well as on porous glass have been investigated. In most cases, [125I]alpha-INF-A was used as an inner standard. The chromatographic behaviour of native and [125I]-labeled alpha-INF-A was practically the same. alpha-INF-A was most effectively chromatographed on porous glass, L-Trp-L-Trp-Sepharose 4B and Cu2+-chelate sorbents. In the latter case, the feasibility of substitution of the Sepharose matrix for the silochrome one has been demonstrated. It has been proposed that alpha-INF-A has a hydrophobic "pocket" with exposed aromatic amino acid residues which are capable of selective binding to aromatic dipeptides.
使用来自产α-干扰素A的细菌菌株的固定化单克隆抗体NK-2进行色谱分离，得到了纯的人白细胞α-干扰素A（α-INF-A），该干扰素在十二烷基硫酸钠存在下进行聚丙烯酰胺凝胶电泳以及反相高效液相色谱时均呈现均一性。研究了部分纯化的α-INF-A在含有固定化染料、芳香二肽、螯合和疏水配体的合成及商业吸附剂以及多孔玻璃上的色谱特性。在大多数情况下，[125I]α-INF-A用作内标。天然和[125I]标记的α-INF-A的色谱行为基本相同。α-INF-A在多孔玻璃、L-色氨酸-L-色氨酸-琼脂糖4B和Cu2+ -螯合吸附剂上的色谱分离效果最佳。在后一种情况下，已证明可以用硅铬基质替代琼脂糖基质。有人提出α-INF-A具有一个疏水的“口袋”，其中暴露的芳香族氨基酸残基能够与芳香二肽选择性结合。
