Arias-Rios Elba Veronica, Tenney Kristina, Mai Tam, Anderson Sam, Cantera Ruth Marie, Nadala Lourdes M, Han Sukkyun, Janagama Harish, Pando Jasmine M, Selover Brandon, Lei Sung Shao, Nadala Cesar, Samadpour Mansour
Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155.
IEH Laboratories and Consulting Group, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155.
J AOAC Int. 2018 Nov 1;101(6):1806-1812. doi: 10.5740/jaoacint.18-0108. Epub 2018 Jul 2.
contamination is a major concern in the ice cream industry; therefore, early and accurate detection is vital. Current detection methods require about a 24 h enrichment period for detection. Enhance the early detection of in ice cream using the highly sensitive isothermal ribosomal RNA-based Roka/Atlas Detection Assay. The R2 Medium was developed for enrichment by Molecular Epidemiology, Inc. (Seattle, WA). Comparative growth curve studies were performed on the new R2 Medium for and the currently validated media for the Roka Detection Assay. Subsequently, a method comparison between the Roka Detection Assay and the U.S. Food and Drug Administration's (FDA) (BAM) Chapter 10 reference method on ice cream was carried out. The R2 Medium supports the growth of better than Buffered Enrichment Broth, Demi-Fraser broth, and Modified University of Vermont Broth, as indicated by the faster growth rate of the organism. When used as an enrichment medium in a method comparison study of ice cream, the results showed that R2 Medium-enriched samples tested with the Roka Detection Assay gave an equivalent performance compared with the 24 h FDA-BAM reference method at 10 and 18 h post-enrichment for . The results from this study indicate that the new R2 Medium and the highly sensitive Roka Detection Assay allowed for the rapid detection of species in ice cream in 13 h. The Roka Detection Assay, in conjunction with a new media formulation (R2 Medium), allowed for the early detection of in ice cream and may be applied in other food matrixes and environmental samples.
污染是冰淇淋行业的一个主要问题；因此，早期准确检测至关重要。目前的检测方法需要约24小时的富集期来进行检测。使用基于核糖体RNA的高灵敏度等温Roka/Atlas检测法提高冰淇淋中[目标物]的早期检测。R2培养基是由分子流行病学公司（华盛顿州西雅图）开发用于[目标物]富集的。对用于[目标物]的新R2培养基和目前已验证的用于Roka检测法的培养基进行了比较生长曲线研究。随后，对Roka检测法与美国食品药品监督管理局（FDA）的[具体方法]（BAM）第10章中关于冰淇淋的参考方法进行了方法比较。R2培养基比缓冲蛋白胨水、半 Fraser肉汤和改良佛蒙特大学肉汤更能支持[目标物]的生长，这体现在该生物体更快的生长速率上。在冰淇淋的方法比较研究中用作富集培养基时，结果表明，用Roka检测法检测经R2培养基富集的样品，在富集后10小时和18小时时，与FDA - BAM参考方法24小时的检测性能相当。本研究结果表明，新的R2培养基和高灵敏度的Roka检测法能够在13小时内快速检测出冰淇淋中的[目标物]种类。Roka检测法与新的培养基配方（R2培养基）相结合，能够早期检测出冰淇淋中的[目标物]，并且可能应用于其他食品基质和环境样品中。
