Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Departments of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Int J Biol Macromol. 2018 Oct 15;118(Pt A):1045-1054. doi: 10.1016/j.ijbiomac.2018.06.160. Epub 2018 Jun 30.
The aims of the current study were to evaluate the antibacterial effects of cellulose nanofiber (CNF), chitosan nanofiber (ChNF), CNF/ChNF combination and chitosan alone (Ch) against the isolated bacterial contaminations from the surface of Iranian banknotes using biochemical, disc diffusion and molecular analyses. The results unveiled that the CNF did not show the significant antibacterial effect against isolated bacterial strains, whereas the combination of CNF/ChNF (at concentration 100 μg/disc 1:1) exhibited the synergistic effects against Stenotrophomonas maltophilia. Also, ChNF (100 μg/disc) and Ch (100 and 200 μg/disc) displayed dose-dependent antibacterial effects against the sensitive bacteria including Bacillus subtilis, Bacillus pumilus, Micrococcus sp. Kosakonia cowanii, Brevibacterium frigoritolerans, Escherichia coli standard ATCC 25922, Staphylococcus aureus standard ATCC 25923. On the other hand, chitosan displayed the highest inhibitory effects against Gram-positive bacteria. Our results showed that the coating of banknotes with these compounds is a novel strategy to reduce the bacterial contaminations and increase the durability (or quality) of banknotes, without being toxic to the surrounding environment.
本研究的目的是评估纤维素纳米纤维（CNF）、壳聚糖纳米纤维（ChNF）、CNF/ChNF 混合物和壳聚糖（Ch）单独对抗从伊朗纸币表面分离的细菌污染的抗菌效果，使用生化、圆盘扩散和分子分析方法。结果表明，CNF 对分离的细菌菌株没有显示出显著的抗菌作用，而 CNF/ChNF（浓度为 100μg/disc 1:1）的组合对嗜麦芽寡养单胞菌表现出协同作用。此外，ChNF（100μg/disc）和 Ch（100 和 200μg/disc）对包括枯草芽孢杆菌、解淀粉芽孢杆菌、微球菌 Kosakonia cowanii、耐冷短杆菌、大肠杆菌标准 ATCC 25922、金黄色葡萄球菌标准 ATCC 25923 在内的敏感细菌表现出剂量依赖性的抗菌作用。另一方面，壳聚糖对革兰氏阳性菌显示出最高的抑制作用。我们的结果表明，用这些化合物涂覆钞票是减少细菌污染和提高钞票耐久性（或质量）的一种新策略，而且对周围环境没有毒性。
