Ohara Kazuaki, Katayama Mikio, Nagai Katsuya
Research Laboratories for Health Science and Food Technologies, Kirin Company, Limited, Yokohama, Kanagawa, 236-0004, Japan.
Research Laboratories for Health Science and Food Technologies, Kirin Company, Limited, Yokohama, Kanagawa, 236-0004, Japan.
Neurosci Lett. 2018 Sep 25;684:18-24. doi: 10.1016/j.neulet.2018.06.057. Epub 2018 Jun 30.
The autonomic nervous system innervates various peripheral tissue functions. Various external stimuli affect autonomic nerve activity, however, there is little information about the involvement of sensory receptors in the responses. The TRPA1 is a calcium-permeable non-selective cation channel which plays a crucial role in the susceptibility to various stimuli. β-Eudesmol, an oxygenated sesquiterpene found in hop essential oil and beer, activates the TRPA1. Intragastric administration of β-eudesmol decreased efferent adrenal sympathetic nerve activity (ASNA) in rats, whereas subcutaneous administration did not. ASNA suppression by β-eudesmol was not observed in TRPA1 knockout rats. The β-eudesmol derived ASNA suppression was partially, but significantly, eliminated by subdiaphragmatic vagotomy in rats, suggesting the afferent vagal nerve from the gastrointestinal tract to the brain is involved in the effect of β-eudesmol on ASNA. Our results indicate that β-eudesmol suppresses ASNA, partly through TRPA1 and the afferent vagus nerve. These findings introduce the physiological significance of the TRPA1 in the control of ASNA.
自主神经系统支配着各种外周组织的功能。各种外部刺激会影响自主神经活动，然而，关于感觉受体在这些反应中的参与情况，目前所知甚少。TRPA1是一种钙通透性非选择性阳离子通道，在对各种刺激的敏感性中起关键作用。β-桉叶醇是啤酒花精油和啤酒中发现的一种含氧倍半萜烯，可激活TRPA1。大鼠胃内给予β-桉叶醇可降低传出肾上腺交感神经活动（ASNA），而皮下给药则无此作用。在TRPA1基因敲除大鼠中未观察到β-桉叶醇对ASNA的抑制作用。大鼠膈下迷走神经切断术可部分但显著消除β-桉叶醇引起的ASNA抑制，这表明从胃肠道到大脑的传入迷走神经参与了β-桉叶醇对ASNA的影响。我们的结果表明，β-桉叶醇部分通过TRPA1和传入迷走神经抑制ASNA。这些发现揭示了TRPA1在ASNA调控中的生理意义。
