Center for Preventive Medical Sciences, Chiba University, Inage-ku Yayoi-cho 1-33, Chiba, 263-8522, Japan.
Center for Marine Environmental Studies, Ehime University, Bunkyo-cho 2-5, Matsuyama, Ehime, 790-8577, Japan.
Environ Pollut. 2018 Nov;242(Pt A):98-105. doi: 10.1016/j.envpol.2018.06.071. Epub 2018 Jun 26.
Lead poisoning is considered a public health threat, particularly in developing countries. Health problems from Pb exposure occur in many parts of the world, especially near Pb mines, Pb smelters, and used lead-acid battery (ULAB) recycling plants. In this study, we analyzed the urine metabolome of residents in a village located near a ULAB recycling facility to investigate the biological effects of Pb exposure (ULAB: n = 44, Reference: n = 51). Lasso linear regression models were moderately predictive of blood Pb levels, as evaluated by a training set (R = 0.813) and against an external test set (R = 0.647). In lasso logistic regression models, areas under receiver operating characteristic curves, as measured by 5-fold cross-validation (AUC = 0.871) and against an external test set (AUC = 0.917), indicated accurate classification of urine samples from the affected village and from a reference site. Ten candidate biomarkers identified at false discovery rates of <0.05 were associated with ATP-binding cassette (ABC) transporters, possibly related to the disruption of small-molecule transport in the kidney; amino acid, porphyrin, and chlorophyll metabolism; and the heme biosynthetic pathway. Collectively, the results suggest that lead Pb is related to the health effects in individuals residing in ULAB site by alteration of these biological pathways.
铅中毒被认为是一种公共卫生威胁，特别是在发展中国家。在世界许多地方，特别是在铅矿、铅冶炼厂和使用过的铅酸电池 (ULAB) 回收厂附近，都存在因 Pb 暴露而导致的健康问题。在这项研究中，我们分析了位于 ULAB 回收设施附近的一个村庄居民的尿液代谢组，以调查 Pb 暴露的生物效应（ULAB：n=44，参考：n=51）。套索线性回归模型对血 Pb 水平具有中等的预测能力，通过训练集评估（R=0.813）和外部测试集（R=0.647）。在套索逻辑回归模型中，通过 5 倍交叉验证（AUC=0.871）和外部测试集（AUC=0.917）测量的接收器操作特征曲线下的面积表明，受影响村庄和参考点的尿液样本得到了准确分类。在错误发现率 <0.05 时确定的 10 个候选生物标志物与 ABC 转运蛋白有关，这可能与肾脏中小分子转运的中断有关；氨基酸、卟啉和叶绿素代谢；和血红素生物合成途径。总的来说，这些结果表明，铅 Pb 通过改变这些生物途径与居住在 ULAB 地点的个体的健康影响有关。
