Coherent Light and Atomic and Molecular Spectroscopy Laboratory, College of Physics, Jilin University, Changchun 130012, PR China.
School of Science, Changchun University of Science and Technology, Changchun 120022, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Nov 5;204:440-445. doi: 10.1016/j.saa.2018.06.061. Epub 2018 Jun 18.
Fluorescence and absorption characteristics of Chlorophyll a (Chl-a) were modulated by the carotenoids (Cars) with different numbers of conjugated carbon‑carbon double bonds in solutions. The Chl-a absorption appears the redshift phenomenon with the effective conjugated of Cars increasing. The absorption of Chl-a and Cars are linearly dependent on intrinsic factors, namely effective conjugate length and functional groups, and on environmental factors, such as the polarizability of the solvent. Cars can be able to quench the Chl-a fluorescence by producing the non-emitting exciplex intermediate. The effective conjugated length of Cars is one of the reasons that effect the fluorescence quenching of Chl-a. According to the Stern-Volmer plots, the Chl-a fluorescence quenching should be predominantly dynamic rather than static.
叶绿素 a（Chl-a）的荧光和吸收特性受溶液中具有不同数量共轭碳-碳双键的类胡萝卜素（Cars）的调节。随着 Cars 的有效共轭增加，Chl-a 的吸收出现红移现象。Chl-a 和 Cars 的吸收与内在因素（即有效共轭长度和官能团）以及环境因素（如溶剂的极化率）线性相关。Cars 可以通过产生非辐射的激基复合物中间体来猝灭 Chl-a 的荧光。Cars 的有效共轭长度是影响 Chl-a 荧光猝灭的原因之一。根据 Stern-Volmer 图，Chl-a 的荧光猝灭应该主要是动态的而不是静态的。
