Institute for Advanced Interdisciplinary Research, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong Province, China.
Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, Shandong Province, China.
Biosens Bioelectron. 2018 Oct 15;117:429-435. doi: 10.1016/j.bios.2018.06.034. Epub 2018 Jun 20.
The accurate detection of the superoxide anion (O) has vital academic and medical diagnostic significance due to its important dual roles in biological functioning. In this work, hollow porous PtAg nanospheres (PtAg HPNSs) were fabricated by a facile hydrothermal method followed by a dealloying procedure. The as-made PtAg nanospheres possessed hollow interiors and porous shells composed of interconnected ligaments and pores with the typical size around 4 nm. Benefitting from the unique hollow nanoporous architecture and the specific alloying effect, the PtAg HPNSs showed high electrocatalytic activity towards superoxide anions. The constructed biosensor based on PtAg HPNSs presented a fast and ultrasensitive response in a wide range of 0.8-1080 nM with much higher sensitivity of 4.5 × 10 μA cm nM and low detection limit of 0.2 nM (S/N = 3). Moreover, the novel biosensors can achieve electrochemical detection for O released from living cells, exhibiting outstanding real time detection capability in cell environment. The facile controllable fabrication and unique sensing performance for PtAg HPNSs offers potential practical applications in developing highly sensitive and stable biosensor towards superoxide anion.
由于超氧阴离子 (O) 在生物功能中具有重要的双重作用，因此准确检测超氧阴离子具有重要的学术和医学诊断意义。在这项工作中，通过简便的水热法和脱合金处理制备了中空多孔 PtAg 纳米球 (PtAg HPNSs)。所制备的 PtAg 纳米球具有由相互连接的链节和孔组成的中空内部和多孔壳，其典型尺寸约为 4nm。受益于独特的中空纳米多孔结构和特定的合金效应，PtAg HPNSs 对超氧阴离子表现出高电催化活性。基于 PtAg HPNSs 构建的生物传感器在 0.8-1080 nM 的宽范围内表现出快速和超灵敏的响应，具有更高的灵敏度 4.5×10 μA cm nM 和低检测限 0.2 nM（S/N=3）。此外，新型生物传感器可以实现对活细胞释放的 O 的电化学检测，在细胞环境中表现出出色的实时检测能力。PtAg HPNSs 的简便可控制造和独特的传感性能为开发对超氧阴离子具有高灵敏度和稳定性的生物传感器提供了潜在的实际应用。
