Department of Breast Care Centre, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510080, China.
College of Chemistry and Materials Science, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China.
Mikrochim Acta. 2023 May 22;190(6):233. doi: 10.1007/s00604-023-05802-2.
Mulberry-like AuPtAg porous hollow nanorods (PHNR) were facilely synthesized for the first time via a wet chemical method, where Au nanorods (Au NR) behaved as sacrificed template. The anisotropic oriented growth and etching process are involved in this synthesis. Their structural and electronic characteristics were scrutinously examined by TEM, EDS, XPS, and electrochemical techniques. The AuPtAg PHNR provided a large specific surface area and exposed a large number of active sites, showing highly enhanced catalytic activity. On this foundation, a label-free electrochemical immunosensor was developed for myoglobin (Myo) assay based on the AuPtAg PHNR. Further, the built sensor exhibited fast and ultrasensitive responses in a linear range of 0.0001 ~ 1000 ng mL with a low limit of detection (LOD = 0.46 pg mL, S/N = 3), and enabled efficient application to human serum samples with acceptable results. Consequently, the developed AuPtAg PHNR-based platform has a broad prospect in practically monitoring Myo and other biomarkers in clinics.
首次通过湿化学法简便地合成了具有类似桑叶的 AuPtAg 多孔空心纳米棒（PHNR），其中 Au 纳米棒（AuNR）作为牺牲模板。该合成涉及各向异性的生长和刻蚀过程。通过 TEM、EDS、XPS 和电化学技术仔细研究了它们的结构和电子特性。AuPtAg PHNR 提供了较大的比表面积并暴露出大量的活性位点，表现出了高度增强的催化活性。在此基础上，基于 AuPtAg PHNR 构建了一种用于肌红蛋白（Myo）检测的无标记电化学免疫传感器。此外，所构建的传感器在 0.0001 至 1000ng/mL 的线性范围内具有快速和超灵敏的响应，检测限低至 0.46pg/mL（S/N=3），并可有效地应用于人体血清样品，结果令人满意。因此，基于开发的 AuPtAg PHNR 平台在临床上实际监测 Myo 和其他生物标志物方面具有广阔的前景。
