Department of Cardiovascular Surgery, Liaocheng People's Hospital, Liaocheng, Shandong Province, China.
Department of Cardiovascular Surgery, Liaocheng People's Hospital, Liaocheng, Shandong Province, China.
Biomed Pharmacother. 2018 Oct;106:303-308. doi: 10.1016/j.biopha.2018.06.090. Epub 2018 Jun 29.
Our aim was to investigate the role of interleukin-6 (IL-6) in myocardial regeneration from mice after cardiac injury. The newborn mice were divided into the following 4 groups (16 in each group): sham group, model group, IL-6-/- group (IL-6 knockout) and IL-6 group (IL-6 overexpression). Electrocardiography was performed on all mice and found higher LVEDD, LVESD and IVST and lower LVEF and LVFS in the IL-6 group compared with the sham group. Using HE staining, severe myocardial injury combined with infarction and fibrosis were observed in the IL-6-/- group, while the damaged myocardial tissue was repaired to some extent in the IL-6 group. The expression of IL-6 in the IL-6 group were significantly up-regulated. BrdU immunofluorescence found that the IL-6-/- group had the least number of BrdU positive cells, while the IL-6 group had more BrdU positive cells than the model group and the IL-6-/- group. Expressions of IL-6, cyclinD1 and Bcl-2 in the IL-6 group were up-regulated compared with other groups. In conclusion, IL-6 overexpression could enhance cardiomyocyte proliferation and relevant protein expression in mice myocardium, thus promoting cardiac regeneration.
我们的目的是研究白细胞介素-6（IL-6）在心脏损伤后小鼠心肌再生中的作用。新生小鼠分为以下 4 组（每组 16 只）：假手术组、模型组、IL-6-/-组（IL-6 基因敲除）和 IL-6 组（IL-6 过表达）。对所有小鼠进行心电图检查，发现 IL-6 组的 LVEDD、LVESD 和 IVST 较高，而 LVEF 和 LVFS 较低。通过 HE 染色，IL-6-/-组观察到严重的心肌损伤合并梗死和纤维化，而 IL-6 组受损的心肌组织在一定程度上得到修复。IL-6 组的 IL-6 表达明显上调。BrdU 免疫荧光发现，IL-6-/-组的 BrdU 阳性细胞数最少，而 IL-6 组的 BrdU 阳性细胞数多于模型组和 IL-6-/-组。与其他组相比，IL-6 组的 IL-6、cyclinD1 和 Bcl-2 表达上调。总之，IL-6 过表达可增强小鼠心肌细胞的增殖和相关蛋白表达，从而促进心脏再生。
