Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China.
Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
Biomed Pharmacother. 2018 Oct;106:342-348. doi: 10.1016/j.biopha.2018.06.143. Epub 2018 Jul 11.
The incidence of nasopharyngeal carcinoma (NPC) in China is relatively higher than that throughout the rest of the world, and NPC is geographically distributed. Long non-coding RNA (lncRNA) plays a key role in the development of tumors. Recent studies have found that the lncRNA Ewing sarcoma-associated transcript 1 (EWSAT1) is highly expressed in various tumors and also in NPCs. The isoflavone calycosin, which is a typical Chinese herbal medicine, can inhibit the growth of breast cancer, colorectal cancer, osteosarcoma and other cancers. The aim of our study was to select NPCs that were sensitive to calycosin and whether calycosin had an effect on NPC cells. If it does, are the effects related to a high expression of EWSAT1? We also verified that EWSAT1 was highly expressed in NPC cells. At the same time, we found that calycosin inhibited the growth of NPC cell lines. To further determine whether the effect of calycosin on NPC cells was related to EWSAT1, we used NPC cells with different concentrations of calycosin and found that the expression of EWSAT1 decreased significantly with increasing concentrations of calycosin and that the expression of downstream factors and pathways were also affected. It was demonstrated that calycosin affected NPC cell growth by regulating EWSAT1 and its downstream pathway. In addition, we overexpressed EWSAT1 and found that the increased expression of EWSAT1 weakened the growth inhibitory effect of calycosin on NPC cells.
鼻咽癌（NPC）在中国的发病率相对高于世界其他地区，并且 NPC 具有地理分布特征。长链非编码 RNA（lncRNA）在肿瘤的发展中起关键作用。最近的研究发现，尤文肉瘤相关转录本 1（EWSAT1）在各种肿瘤中高表达，也在 NPC 中高表达。染料木黄酮是一种典型的中草药，它可以抑制乳腺癌、结直肠癌、骨肉瘤等癌症的生长。我们的研究目的是选择对染料木黄酮敏感的 NPC 细胞，以及染料木黄酮是否对 NPC 细胞有作用。如果有作用，这些作用是否与 EWSAT1 的高表达有关？我们还验证了 EWSAT1 在 NPC 细胞中高表达。同时，我们发现染料木黄酮抑制 NPC 细胞系的生长。为了进一步确定染料木黄酮对 NPC 细胞的作用是否与 EWSAT1 有关，我们使用不同浓度染料木黄酮的 NPC 细胞，发现随着染料木黄酮浓度的增加，EWSAT1 的表达明显下降，下游因子和途径的表达也受到影响。结果表明，染料木黄酮通过调节 EWSAT1 及其下游通路影响 NPC 细胞的生长。此外，我们过表达 EWSAT1，发现 EWSAT1 表达增加削弱了染料木黄酮对 NPC 细胞生长抑制作用。
