a Department of Otorhinolaryngology , Hangzhou First People's Hospital , Hangzhou , PR China.
b Department of Pathology , Yancheng Hospital Affiliated Southeast University , Yancheng , PR China.
J Drug Target. 2019 Aug;27(7):806-811. doi: 10.1080/1061186X.2018.1561887. Epub 2019 Feb 11.
Nasopharyngeal carcinoma (NPC) is the most common aggressive squamous cell carcinoma in head and neck cancers. Although advanced radiochemotherapy has been applied, the prognosis of NPC patients still remains poor due to the regional relapse and distant metastasis. Polyphyllin I (PP I) is extracted from natural herb that has been widely used in cancer treatment and long non-coding RNA (lncRNA) has been reported to play a key role in the anti-tumour activity of PP I. In this study, it has been found that PP I inhibited the proliferation and induced apoptosis of NPC cells in a dose-dependent manner. A higher level of lncRNA regulator of reprogramming (ROR) expression was detected in NPC cell lines compared with normal nasopharyngeal cell line and PP I must significantly down-regulate the expression level of lncRNA ROR. LncRNA ROR/P53 signalling was essential for PP I-suppressed NPC progression. These data indicated that PP I suppressed tumour growth and induced apoptosis of NPC and through down-regulation of lncRNA-ROR, subsequently upregulating of P53 signalling. LncRNA ROR may be a potential therapeutic target and PP I would be a promising candidate for NPC treatment.
鼻咽癌（NPC）是头颈部癌症中最常见的侵袭性鳞状细胞癌。尽管已经应用了先进的放化疗，但由于区域性复发和远处转移，NPC 患者的预后仍然较差。重楼皂苷 I（PP I）是从天然草药中提取的，已广泛用于癌症治疗，并且长链非编码 RNA（lncRNA）已被报道在 PP I 的抗肿瘤活性中起关键作用。在这项研究中，已经发现 PP I 以剂量依赖性方式抑制 NPC 细胞的增殖并诱导其凋亡。与正常鼻咽细胞系相比，NPC 细胞系中检测到更高水平的 lncRNA 重编程调节因子（ROR）表达，而 PP I 必须显著下调 lncRNA ROR 的表达水平。lncRNA ROR/P53 信号通路对于 PP I 抑制的 NPC 进展至关重要。这些数据表明，PP I 通过下调 lncRNA-ROR 抑制肿瘤生长并诱导 NPC 细胞凋亡，从而上调 P53 信号通路。lncRNA ROR 可能是一个潜在的治疗靶点，PP I 可能是 NPC 治疗的有前途的候选药物。
