检测转录组中的 RNA G-四链体 (rG4s)。
Detecting RNA G-Quadruplexes (rG4s) in the Transcriptome.
机构信息
Department of Chemistry, City University of Hong Kong, Hong Kong SAR, China.
Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.
出版信息
Cold Spring Harb Perspect Biol. 2018 Jul 2;10(7):a032284. doi: 10.1101/cshperspect.a032284.
Abstract
RNA G-quadruplex (rG4) secondary structures are proposed to play key roles in fundamental biological processes that include the modulation of transcriptional, co-transcriptional, and posttranscriptional events. Recent methodological developments that include predictive algorithms and structure-based sequencing have enabled the detection and mapping of rG4 structures on a transcriptome-wide scale at high sensitivity and resolution. The data generated by these studies provide valuable insights into the potentially diverse roles of rG4s in biology and open up a number of mechanistic hypotheses. Herein we highlight these methodologies and discuss the associated findings in relation to rG4-related biological mechanisms.
摘要
RNA 四链体 (rG4) 二级结构被认为在包括转录、共转录和转录后事件调节等基本生物过程中发挥关键作用。最近的方法学发展，包括预测算法和基于结构的测序，使得能够在高灵敏度和分辨率下在全转录组范围内检测和绘制 rG4 结构。这些研究产生的数据为 rG4 在生物学中的潜在多样化作用提供了有价值的见解，并提出了许多机制假设。本文中，我们重点介绍了这些方法，并讨论了与 rG4 相关的生物学机制相关的发现。
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