• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用特异性酶抑制剂125I-351A对大鼠单个脑细胞核中血管紧张素转换酶（激肽酶II）结合进行定量放射自显影测定。

Quantitative autoradiographic determination of angiotensin-converting enzyme (kininase II) binding in individual rat brain nuclei with 125I-351A, a specific enzyme inhibitor.

作者信息

Correa F M, Plunkett L M, Saavedra J M, Hichens M

出版信息

Brain Res. 1985 Nov 11;347(1):192-5. doi: 10.1016/0006-8993(85)90912-6.

DOI:10.1016/0006-8993(85)90912-6
PMID:2996715
Abstract

We report the localization and characterization of angiotensin-converting enzyme (kininase II) in discrete nuclei from individual rat brains by a quantitative autoradiographic technique coupled to computerized microdensitometry. The enzyme was quantitated by incubation of 16-micron-thick brain sections with 0.07-2 nM of the converting enzyme inhibitor 125I-351A and comparison to 125I-standards. This technique can be applied to the study of other enzymes in single rat brain nuclei.

摘要

我们通过结合计算机微密度测定法的定量放射自显影技术，报告了从单个大鼠大脑的离散核中血管紧张素转换酶（激肽酶II）的定位和特征。通过将16微米厚的脑切片与0.07 - 2 nM的转换酶抑制剂125I - 351A孵育，并与125I标准品进行比较来定量该酶。该技术可应用于对单个大鼠脑核中其他酶的研究。

相似文献

1
Quantitative autoradiographic determination of angiotensin-converting enzyme (kininase II) binding in individual rat brain nuclei with 125I-351A, a specific enzyme inhibitor.用特异性酶抑制剂125I-351A对大鼠单个脑细胞核中血管紧张素转换酶（激肽酶II）结合进行定量放射自显影测定。
Brain Res. 1985 Nov 11;347(1):192-5. doi: 10.1016/0006-8993(85)90912-6.
2
Quantitative distribution of angiotensin-converting enzyme (kininase II) in discrete areas of the rat brain by autoradiography with computerized microdensitometry.通过放射自显影术结合计算机微密度测定法对大鼠脑离散区域中血管紧张素转换酶（激肽酶II）进行定量分布研究。
Brain Res. 1986 Jun 11;375(2):259-66. doi: 10.1016/0006-8993(86)90746-8.
3
Autoradiography of angiotensin-converting enzyme in fixed and unfixed rat brain using the specific enzyme inhibitor [125I]351A or a polyclonal antibody and [125I]staphylococcal protein A.使用特异性酶抑制剂[125I]351A或多克隆抗体及[125I]葡萄球菌蛋白A对固定和未固定的大鼠脑内血管紧张素转换酶进行放射自显影。
Neurosci Lett. 1990 Mar 14;110(3):244-8. doi: 10.1016/0304-3940(90)90854-3.
4
Axonal transport of angiotensin-converting enzyme in the rat striatonigral pathway.血管紧张素转换酶在大鼠纹状体黑质通路中的轴突运输
Brain Res. 1988 Apr 26;447(1):195-9. doi: 10.1016/0006-8993(88)90986-9.
5
Comparative quantification of rat brain and pituitary angiotensin-converting enzyme with autoradiographic and enzymatic methods.用放射自显影法和酶法对大鼠脑和垂体血管紧张素转换酶进行比较定量分析。
Brain Res. 1991 Apr 5;545(1-2):215-22. doi: 10.1016/0006-8993(91)91289-d.
6
Angiotensin converting enzyme in rat brain visualized by quantitative in vitro autoradiography.通过定量体外放射自显影法观察大鼠脑中的血管紧张素转换酶。
Neuroscience. 1987 Feb;20(2):615-27. doi: 10.1016/0306-4522(87)90114-x.
7
Two binding sites on angiotensin-converting enzyme: evidence from radioligand binding studies.血管紧张素转换酶上的两个结合位点：放射性配体结合研究的证据。
Mol Pharmacol. 1992 Aug;42(2):286-93.
8
Quantitative autoradiographic determination of angiotensin-converting enzyme binding in rat pituitary and adrenal glands with 125I-351A, a specific inhibitor.
Regul Pept. 1985 Nov 28;12(4):263-72. doi: 10.1016/0167-0115(85)90169-7.
9
In vitro autoradiographic localization of angiotensin-converting enzyme in rat brain using 125I-labelled MK351A.
J Hypertens Suppl. 1984 Dec;2(3):S41-4.
10
Angiotensin II receptor subtypes and angiotensin-converting enzyme in the fetal rat brain.
Brain Res. 1993 Dec 24;631(2):212-20. doi: 10.1016/0006-8993(93)91537-3.

引用本文的文献

1
Effect of NMDA-induced lesion of the subfornical organ on the angiotensin II binding sites density and acetylcholinesterase or NADPH-diphorase activities in the lamina terminalis of the rat brain.N-甲基-D-天冬氨酸诱导的穹窿下器官损伤对大鼠脑终板血管器中血管紧张素II结合位点密度及乙酰胆碱酯酶或还原型辅酶II-黄递酶活性的影响
Cell Mol Neurobiol. 2001 Feb;21(1):81-90. doi: 10.1023/a:1007125413308.
2
Angiotensin converting enzyme inhibitors as potential cognitive enhancing agents.血管紧张素转换酶抑制剂作为潜在的认知增强剂。
J Psychiatry Neurosci. 1994 Jan;19(1):46-50.
3
Effects of deoxycorticosterone acetate (DOCA) and aldosterone on Sar1-angiotensin II binding and angiotensin-converting enzyme binding sites in brain.
醋酸脱氧皮质酮（DOCA）和醛固酮对大脑中Sar1-血管紧张素II结合位点及血管紧张素转换酶结合位点的影响。
Cell Mol Neurobiol. 1993 Oct;13(5):529-39. doi: 10.1007/BF00711461.
4
Radioimmunochemical methods for the quantitative autoradiographic determination of antigens in brain and other tissues.用于脑及其他组织中抗原定量放射自显影测定的放射免疫化学方法。
Cell Mol Neurobiol. 1988 Mar;8(1):57-70. doi: 10.1007/BF00712912.
5
The kallikrein-kinin system in the rat hypothalamus. Immunohistochemical localization of high molecular weight kininogen and T kininogen in different neuronal systems.大鼠下丘脑的激肽释放酶-激肽系统。高分子量激肽原和T激肽原在不同神经元系统中的免疫组织化学定位。
Histochemistry. 1991;96(3):229-43. doi: 10.1007/BF00271541.