Correa F M, Plunkett L M, Saavedra J M
Brain Res. 1986 Jun 11;375(2):259-66. doi: 10.1016/0006-8993(86)90746-8.
We report the localization of angiotensin-converting enzyme (kininase II, EC 3.4.15.1) in discrete nuclei and areas of the rat brain by a quantitative autoradiographic technique using image processing coupled to computerized microdensitometry, after incubation of brain sections with the specific converting enzyme inhibitor [125I]351A. High angiotensin-converting enzyme levels are present in circumventricular organs (organon subfornicalis and area postrema), the choroid plexus, and extrapyramidal areas (nucleus caudatus, globus pallidus and substantia nigra) with intermediate levels in selected hypothalamic, septal, habenular and brainstem nuclei. Our results support the idea that angiotensin II could be formed in specific brain areas, both outside and inside the blood-brain barrier. In other brain structures, such as the extrapyramidal areas, kininase II could be involved in the processing or metabolism of other brain peptides.
我们通过定量放射自显影技术报告了血管紧张素转换酶（激肽酶II，EC 3.4.15.1）在大鼠脑离散核团和区域中的定位，该技术采用图像处理与计算机微密度测定相结合的方法，在脑切片与特异性转换酶抑制剂[125I]351A孵育后进行。血管紧张素转换酶水平高的部位存在于室周器官（穹窿下器和最后区）、脉络丛和锥体外系区域（尾状核、苍白球和黑质），而在选定的下丘脑、隔区、缰核和脑干核团中水平中等。我们的结果支持这样一种观点，即血管紧张素II可能在血脑屏障内外的特定脑区形成。在其他脑结构中，如锥体外系区域，激肽酶II可能参与其他脑肽的加工或代谢。
