高效酵母转化
High-Efficiency Yeast Transformation.
作者信息
Reece-Hoyes John S, Walhout Albertha J M
出版信息
Cold Spring Harb Protoc. 2018 Jul 2;2018(7):2018/7/pdb.prot094995. doi: 10.1101/pdb.prot094995.
PMID:29967274
Abstract
High-efficiency yeast transformation is used for integrations into YM4271 (yeast one-hybrid (Y1H) DNA-bait generation), for transforming libraries of activation domain (AD)-prey clones into Y1H and yeast two-hybrid (Y2H)-bait strains, and for gap repair. The protocol takes 2 d to complete.
摘要
高效酵母转化用于整合到YM4271（酵母单杂交（Y1H）DNA诱饵生成）中，用于将激活域（AD）-猎物克隆文库转化到Y1H和酵母双杂交（Y2H）-诱饵菌株中，以及用于缺口修复。该方案需要2天完成。
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