Jin Fulai, Avramova Larisa, Huang Jing, Hazbun Tony
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.
Nat Methods. 2007 May;4(5):405-7. doi: 10.1038/nmeth1042. Epub 2007 Apr 22.
We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.
我们在此展示一种新型的双杂交智能池阵列（SPA）系统，在该系统中，不是使用单个激活域菌株，而是以阵列形式筛选精心设计的激活域池，这种阵列形式允许内置复制和猎物-诱饵反卷积。使用这种方法，酿酒酵母基因组SPA将酵母双杂交筛选效率提高了一个数量级。
