基于 SCAR 标记的柑橘靶斑病病原菌检测诊断方法的建立。
Development of a SCAR Marker-Based Diagnostic Method for the Detection of the Citrus Target Spot Pathogen .
机构信息
College of Plant Protection, Southwest University, Chongqing 400715, China.
Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, College Park, MD 20850, USA.
出版信息
Biomed Res Int. 2018 Jun 3;2018:7128903. doi: 10.1155/2018/7128903. eCollection 2018.
Target spot, a recently observed citrus disease that is caused by , can cause substantial economic losses in citrus production. In this study, a 797 bp marker specific to was identified via random amplified polymorphic DNA (RAPD) technique. The primer pair Pc-SFP/Pc-SRP, which was designed from RAPD amplicons, was utilized as a sequence-characterized amplified region (SCAR) marker. This marker identified with a single and distinct band of 389 bp but did not amplify DNA from other tested fungal species. The PCR assay was highly sensitive to the target DNA at picogram levels and could reliably amplify sequences with the Pc-SFP/Pc-SRP primer pair. The SCAR marker that was identified in the present study can facilitate rapid decision-making and precise disease forecasting and management.
靶标斑，一种最近观察到的由 引起的柑橘病害，可能会给柑橘生产造成巨大的经济损失。本研究利用随机扩增多态性 DNA (RAPD)技术，鉴定出一个与 相关的 797bp 的特异标记。根据 RAPD 扩增子设计的引物对 Pc-SFP/Pc-SRP 被用作序列特征扩增区域 (SCAR)标记。该标记可特异性识别出 389bp 的单一且独特的条带，但不能扩增来自其他测试真菌物种的 DNA。PCR 检测法对靶 DNA 的灵敏度可达皮克级水平，并且可以使用 Pc-SFP/Pc-SRP 引物对可靠地扩增 序列。本研究中鉴定的 SCAR 标记可促进快速决策以及精准的疾病预测和管理。
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