School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Infinitus Chinese Herbal Immunity Research Centre, Guangzhou 510600, China.
J Immunol Res. 2018 Jun 3;2018:3431782. doi: 10.1155/2018/3431782. eCollection 2018.
polysaccharide (LBP) is isolated from the fruit of Chinese herbal . Previous studies had demonstrated that LBP could inhibit tumor growth and enhance the immunity in mice. However, the effect of LBP on systemic and local immune responses , especially on phenotypic and functional changes of T cells, is still largely unknown. In the present study, we investigated the effects of LBP on systemic and local T cell-dependent antitumor immune responses in H22 tumor-bearing mice. The results showed that LBP could inhibit the solid tumor growth in mice, but showed little effect on the body weight or spleen index. Furthermore, LBP could maintain high levels of T cells in peripheral blood (PB), tumor draining lymph node (TDLN), and tumor tissue, prevent the increase of Tregs while promote infiltration of CD8 T cells in tumor tissue, inhibit the production of TGF-1 and IL-10 in serum, decrease the exhaustion phenotype of T cells, and maintain cytotoxicity of lymphocytes. Taken together, our results demonstrated that LBP simultaneously induced systemic and local immune responses in H22 tumor-bearing mice by alleviating immunosuppression and maintaining antitumor immune responses in mice.
多糖（LBP）从中药果实中分离出来。先前的研究表明，LBP 可以抑制肿瘤生长并增强小鼠的免疫力。然而，LBP 对全身和局部免疫应答的影响，特别是对 T 细胞的表型和功能变化的影响，在很大程度上仍不清楚。在本研究中，我们研究了 LBP 对 H22 荷瘤小鼠全身和局部依赖 T 细胞的抗肿瘤免疫应答的影响。结果表明，LBP 可抑制小鼠实体瘤生长，但对体重或脾脏指数影响不大。此外，LBP 可维持外周血（PB）、肿瘤引流淋巴结（TDLN）和肿瘤组织中 T 细胞的高水平，防止 Tregs 的增加，同时促进 CD8 T 细胞在肿瘤组织中的浸润，抑制血清中 TGF-1 和 IL-10 的产生，减少 T 细胞的耗竭表型，并保持淋巴细胞的细胞毒性。总之，我们的结果表明，LBP 通过减轻免疫抑制和维持小鼠的抗肿瘤免疫应答，同时诱导 H22 荷瘤小鼠的全身和局部免疫应答。
