Guo Yindi, Liu Jie, Tuo Qiang, Zhang Dongtao, Wanapat Metha, Xin Guosheng
School of Life Science, Ningxia University, Yinchuan, China.
Ningxia Feed Engineering Technology Research Center, Ningxia University, Yinchuan, China.
Front Vet Sci. 2024 Jul 31;11:1416793. doi: 10.3389/fvets.2024.1416793. eCollection 2024.
This study was conducted to investigate both fruit and different levels of leaf supplementation on the growth performance, organ indices and intestinal microflora of rats. Twenty-five healthy male Sprague-Dawley rats were randomly divided into five groups. The rats in the control (NC) and positive control (PC) groups were fed by gavage a basal diet and a basal diet with 4 g/kg of fruit homogenate, respectively. The test (LD, MD, and HD) groups were fed basal diets with additional 2, 4, and 8 g/kg of leaf homogenate, respectively. The feeding period was 35 d. The result revealed that the rats in the LD group had the highest average weight gain ( < 0.05). The cardiac and renal indexes in the LD and MD groups were significantly higher than in NC group, respectively ( < 0.05). Diversity analysis revealed that adding low concentrations of leaf homogenates markedly reduced the Shannon index of the rats cecum ( < 0.05). The relative abundance of Verrucomicrobiota was higher in the LD group than those in other groups ( < 0.05). The relative abundance of Actinobacteriota was found significantly higher in PC group than others ( < 0.05). The relative abundance of in LD group was the highest ( < 0.05). The relative abundance of Romboutsia in the PC group was considerably higher than that in other groups. The relative abundance of Candidatus_Saccharimonas in the supplementation groups was appreciably lower than those found in other groups. The relative abundance of Alloprevotella was significantly lower in PC, LD, and MD groups than in NC and HD groups ( < 0.05). The relative abundance of Oscillibacter was significantly higher in HD group than in other groups ( < 0.05). Thus, leaf homogenate fed to rats could increase their growth performance, internal organ weights and additionally enhance the relative abundance of beneficial bacteria. Therefore, based on the obtained data in the current study, a dose of leaf homogenate supplemented with 2 g/kg in diet is recommended, however, further studies are required to confirm, especially in animals.
本研究旨在探讨水果及不同水平的叶片补充剂对大鼠生长性能、器官指数和肠道微生物群的影响。将25只健康雄性Sprague-Dawley大鼠随机分为五组。对照组（NC）和阳性对照组（PC）的大鼠分别通过灌胃给予基础日粮和添加4 g/kg水果匀浆的基础日粮。试验组（LD、MD和HD）分别给予添加2、4和8 g/kg叶片匀浆的基础日粮。饲养期为35天。结果显示，LD组大鼠的平均体重增加最高（<0.05）。LD组和MD组的心脏和肾脏指数分别显著高于NC组（<0.05）。多样性分析表明，添加低浓度叶片匀浆显著降低了大鼠盲肠的香农指数（<0.05）。LD组疣微菌门的相对丰度高于其他组（<0.05）。放线菌门的相对丰度在PC组显著高于其他组（<0.05）。LD组中[具体菌名未给出]的相对丰度最高（<0.05）。PC组中罗氏菌属的相对丰度明显高于其他组。补充剂组中候选糖单胞菌属的相对丰度明显低于其他组。PC组、LD组和MD组中别普雷沃菌属的相对丰度显著低于NC组和HD组（<0.05）。HD组中颤杆菌属的相对丰度显著高于其他组（<0.05）。因此，给大鼠喂食叶片匀浆可提高其生长性能、内脏器官重量，并额外增加有益细菌的相对丰度。因此，根据本研究获得的数据，建议日粮中添加2 g/kg的叶片匀浆剂量，然而，需要进一步研究来证实，尤其是在动物身上。
