小鼠膀胱中嘌呤能传递的证据以及5-羟色胺对电刺激反应的调节作用。
Evidence for purinergic transmission in mouse bladder and for modulation of responses to electrical stimulation by 5-hydroxytryptamine.
作者信息
Holt S E, Cooper M, Wyllie J H
出版信息
Eur J Pharmacol. 1985 Oct 8;116(1-2):105-11. doi: 10.1016/0014-2999(85)90190-6.
Electrical stimulation (ES) contracted superfused mouse bladder, and 10(-7) M tetrodotoxin (TTX) abolished the twitches without impairing responses to acetylcholine (ACh) or beta,gamma-methylene ATP. ES acted largely through nerves which were not cholinergic, adrenergic or histaminergic. They may be purinergic because the bladder was contracted by stable analogues of ATP, and after desensitisation by a high concentration of alpha,beta-methylene ATP the response to ES was selectively reduced. 5-Hydroxytryptamine (5-HT) at 0.03-3 X 10(-6) M and tetraethylammonium (TEA) at 0.1-10 X 10(-3) M potentiated responses to ES, on average by 64% and 182%. Pempidine had no effect on responses to ES. The action of TEA was different from that of 5-HT; potentiation of responses was greater than could be produced by 5-HT, and whereas 5-HT did not increase responses to ACh, TEA markedly increased twitch tensions. The mode of action of 5-HT is not clear.
电刺激（ES）可使灌流的小鼠膀胱收缩，而10⁻⁷ M河豚毒素（TTX）可消除抽搐，同时不损害对乙酰胆碱（ACh）或β,γ-亚甲基ATP的反应。ES主要通过非胆碱能、非肾上腺素能和非组胺能神经起作用。它们可能是嘌呤能神经，因为ATP的稳定类似物可使膀胱收缩，并且在被高浓度的α,β-亚甲基ATP脱敏后，对ES的反应会选择性降低。0.03 - 3×10⁻⁶ M的5-羟色胺（5-HT）和0.1 - 10×10⁻³ M的四乙铵（TEA）可增强对ES的反应，平均分别增强64%和182%。潘必定对ES反应无影响。TEA的作用与5-HT不同；对反应的增强作用大于5-HT所能产生的效果，而且5-HT不会增加对ACh的反应，而TEA则明显增加抽搐张力。5-HT的作用方式尚不清楚。
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