Laboratory of Veterinary Biochemistry and Molecular Biology, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
Laboratory of Veterinary Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, South Korea.
Reprod Toxicol. 2018 Sep;80:60-67. doi: 10.1016/j.reprotox.2018.06.071. Epub 2018 Jun 30.
Humans are at daily risk by simultaneous exposures to a broad spectrum of man-made chemicals in the commercial products. Several classes of chemicals have been shown to alter follicle development and reduce fertility, leading to premature ovarian failure (POF) in mammals. We investigate the synergistic effects of 4-vinylcyclohexene diepoxide (VCD) and phthalate, including di(2-ethylhexyl) phthalate (DEHP), butyl benzyl phthalate (BBP) and di-n-butyl phthalate (DBP) on POF. Combination exposure with VCD and phthalate significantly reduced the numbers of primary follicles. The expressions of Amh and Sohlh2 were significantly decreased in the combination groups. Serum Amh levels were significantly lower in the combination groups. Additionally, serum levels of follicle-stimulating hormone were significantly increased in combination groups. Taken together, exposure to phthalates promotes the depletion of follicular follicles and consequently increases the risk of premature menopause, and combined exposure of phthalates and VCD to early menopausal women is likely to aggravate the POF syndrome.
人类在日常生活中会同时接触到商业产品中广泛存在的人造化学物质。几类化学物质已被证明会改变卵泡发育并降低生育能力，导致哺乳动物的卵巢早衰（POF）。我们研究了 4-乙烯基环己烯二恶烷（VCD）和邻苯二甲酸酯（包括邻苯二甲酸二（2-乙基己基）酯（DEHP）、邻苯二甲酸丁基苄基酯（BBP）和邻苯二甲酸二丁酯（DBP））对 POF 的协同作用。VCD 和邻苯二甲酸酯的联合暴露显著减少了初级卵泡的数量。组合组中 Amh 和 Sohlh2 的表达明显下降。组合组的血清 Amh 水平明显降低。此外，组合组的卵泡刺激素血清水平显著升高。总之，邻苯二甲酸盐的暴露会促进卵泡的耗竭，从而增加过早绝经的风险，而对早期绝经女性同时接触邻苯二甲酸盐和 VCD 可能会加重 POF 综合征。
