Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering , Shaanxi Normal University , Xi'an 710119 , P. R. China.
Anal Chem. 2018 Aug 7;90(15):9629-9636. doi: 10.1021/acs.analchem.8b02577. Epub 2018 Jul 13.
As one of the most efficient and commonly used electrochemiluminescence (ECL) reagents, luminol has been paid much attention by the analysts due to its low excitation potential, simple dissolved oxygen-based coreactant ECL reaction requirement, and the widely analytical applications. However, the ECL performances of luminol on most electrode materials suffered from the lower ECL quantum yield, which limited its analytical applications. Herein, it was first found that, compared to that of the bare gold electrode, the ECL quantum yield of luminol on the 1,6-hexanedithiol hydrophobic pinhole film modified gold electrode was 3 times increased. This higher ECL quantum yield of luminol was related to the hydrophobic microenvironment on the surface of the modified electrode, which was formed from the hydrophobic carbon chains on the basis of their supramolecular interaction. On the basis of this new finding as well as the cap effect of gold nanoparticle to these pinhole gates, a highly sensitive ECL sensing scheme for microRNA was also developed.
作为最有效和常用的电化学发光 (ECL) 试剂之一，由于其低激发电位、简单的基于溶解氧的共反应物 ECL 反应要求以及广泛的分析应用，鲁米诺受到了分析人员的高度关注。然而，鲁米诺在大多数电极材料上的 ECL 性能受到较低的 ECL 量子产率的限制，这限制了其分析应用。本文首次发现，与裸金电极相比，1,6-己二硫醇疏水性微孔膜修饰金电极上鲁米诺的 ECL 量子产率提高了 3 倍。鲁米诺具有更高的 ECL 量子产率与修饰电极表面疏水性微环境有关，该微环境是基于超分子相互作用形成的疏水性碳链。基于这一新发现以及金纳米粒子对这些微孔门的盖帽效应，还开发了一种用于 microRNA 的高灵敏度 ECL 传感方案。
