College of Chemistry and Materials Science, Editorial Department of Journal of Anhui Normal University, Anhui Normal University, Wuhu, 241000, Anhui, China.
Anal Bioanal Chem. 2021 Jul;413(16):4119-4135. doi: 10.1007/s00216-021-03247-1. Epub 2021 Mar 14.
Electrochemiluminescence (ECL) can be produced through two main routes: annihilation route and coreactant route. The vast majority of applications of ECL are based on coreactant ECL which can be generated in aqueous media at relatively low potentials compared with organic solvents. However, the development of more efficient ECL systems remains a compelling goal. Co-reaction accelerator (CRA) can significantly enhance the ECL signal through promoting more production of the coreactant intermediate. Compared with other ECL enhancement strategies, the CRA protocol is distinctive owing to its diverse, simple, and highly effective features. Various species such as inorganic compound, organic compound, and nanomaterials (NMs) have been developed as CRA and NM CRA has gained particular attention owing to their unique properties of excellent catalytic behavior and large surface area. By integration with the inherent advantages of ECL, bioanalysis based on CRA-enhanced ECL showed excellent performance such as ultrahigh sensitivity, wide dynamic range, low cost, simple instrumentation, and measurements in complex media. It has been extensively applied in various fields including clinical diagnosis, environmental monitoring, and food safety. Therefore, it is of great interest to present a systematic and critical review on the advances in ECL CRA. Herein, the recent progress on CRA and its applications in ECL bioanalysis are summarized by illustrating some representative work and a discussion of the future development trends of CRA ECL is offered.
电化学发光 (ECL) 可以通过两种主要途径产生：湮灭途径和共反应途径。与有机溶剂相比，绝大多数 ECL 的应用都基于共反应 ECL，它可以在水介质中在相对较低的电势下产生。然而，开发更有效的 ECL 系统仍然是一个紧迫的目标。共反应加速剂 (CRA) 通过促进更多的共反应中间体的产生，可以显著增强 ECL 信号。与其他 ECL 增强策略相比，CRA 方案具有独特的多样性、简单性和高效性。各种物质如无机化合物、有机化合物和纳米材料 (NMs) 已被开发为 CRA，NM CRA 因其优异的催化行为和大表面积的独特性质而受到特别关注。通过与 ECL 的固有优势相结合，基于 CRA 增强的 ECL 的生物分析表现出超高灵敏度、宽动态范围、低成本、简单的仪器和在复杂介质中的测量等优异性能。它已广泛应用于临床诊断、环境监测和食品安全等各个领域。因此，对 ECL CRA 的进展进行系统和批判性的综述是很有意义的。本文通过举例说明一些代表性工作，总结了 CRA 及其在 ECL 生物分析中的应用的最新进展，并对 CRA ECL 的未来发展趋势进行了讨论。
