四种药用植物的抗菌相互作用、抗炎和细胞毒性作用。
Antibacterial interactions, anti-inflammatory and cytotoxic effects of four medicinal plant species.
机构信息
Department of Biochemistry, Microbiology and Biotechnology, Faculty of Science and Agriculture, University of Limpopo, Turfloop Campus, Private Bag X1106, Sovenga, Limpopo, 0727, South Africa.
Department of Paraclinical Sciences, Phytomedicine Programme, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa.
出版信息
BMC Complement Altern Med. 2018 Jul 3;18(1):199. doi: 10.1186/s12906-018-2264-z.
BACKGROUND
The constant emergence of antibiotic resistant species and the adverse side effects of synthetic drugs are threatening the efficacy of the drugs that are currently in use. This study was aimed at investigating the possible antibacterial interactions, anti-inflammatory and cytotoxic effects of selected medicinal plants based on their traditional usage.
METHODS
The acetone extracts of four plant species were assessed independently and in combination for antibacterial activity using microdilution assay and the sum of the fractional inhibitory concentration (FIC) was calculated. The ability of Dombeya rotundifolia and Schkuhria pinnata extracts to inhibit the production of reactive oxygen species (ROS) in LPS induced RAW 264.7 macrophage cells was evaluated using Dichloro-dihydro-fluorescein diacetate (HDCF-DA) assay to determine anti-inflammatory potential and the toxicity on African green monkey kidney (Vero) cells was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.
RESULTS
The antibacterial efficacies of the different combinations of Schkuhria pinnata (A), Commelina africana (B), Dombeya rotundifolia (C) and Elephantorrhiza elephantina (D) plants varied from combination to combination. Synergistic effects were only exhibited against P. aeruginosa, while the antagonistic effects were only observed against E. coli. Both S. pinnata and D. rotundifolia demonstrated anti-inflammatory potential by inhibiting the production of ROS in a dose dependant manner. The cytotoxicity of the plants (LC values) ranged from < 25.0 to 466.1 μg/mL. S pinnata extract was the most toxic with the lowest LC value of < 25.0 μg/mL.
CONCLUSIONS
The synergistic interaction observed indicates that combinational therapy may improve biological activity. This report highlights the anti-inflammatory potential of S. pinnata and D. rotundifolia; which could be exploited in the search for anti-inflammatory agents. However, the cytotoxicity of S. pinnata highlights the importance of using this plant with caution.
背景
抗生素耐药物种的不断出现和合成药物的不良反应正对现有药物的疗效构成威胁。本研究旨在根据传统用途，研究选定药用植物的潜在抗菌相互作用、抗炎和细胞毒性作用。
方法
分别评估四种植物物种的丙酮提取物的抗菌活性，并使用微量稀释法进行组合评估，计算部分抑菌浓度（FIC）总和。采用二氯二氢荧光素二乙酸酯（HDCF-DA）测定法评估多穗柯（Dombeya rotundifolia）和山蚂蝗（Schkuhria pinnata）提取物抑制脂多糖诱导的 RAW 264.7 巨噬细胞中活性氧（ROS）产生的能力，以确定抗炎潜力，并通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物（MTT）测定法评估对非洲绿猴肾（Vero）细胞的毒性。
结果
不同组合的 Schkuhria pinnata（A）、鸭跖草（Commelina africana）（B）、多穗柯（Dombeya rotundifolia）（C）和象皮树（Elephantorrhiza elephantina）（D）植物的抗菌功效因组合而异。仅对铜绿假单胞菌表现出协同作用，而仅对大肠杆菌表现出拮抗作用。S pinnata 和 D. rotundifolia 均通过剂量依赖性抑制 ROS 的产生显示出抗炎潜力。植物的细胞毒性（LC 值）范围为 <25.0 至 466.1μg/mL。S pinnata 提取物毒性最大，LC 值<25.0μg/mL。
结论
观察到的协同相互作用表明联合治疗可能会提高生物活性。本报告强调了 S. pinnata 和 D. rotundifolia 的抗炎潜力；这可能会被用于寻找抗炎剂。然而，S. pinnata 的细胞毒性强调了谨慎使用该植物的重要性。
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