Leighton Ximena, Bera Alakesh, Eidelman Ofer, Eklund Michael, Puthillathu Narayanan, Pollard Harvey B, Srivastava Meera
Department of Anatomy, Physiology, and Genetics, and Institute for Molecular Medicine, Uniformed Services University School of Medicine (USUHS), Bethesda, MD, U.S.A.
Anticancer Res. 2018 Jul;38(7):3831-3842. doi: 10.21873/anticanres.12667.
BACKGROUND/AIM: Our studies showed that ANXA7 is a novel tumor suppressor gene that is lost in various aggressive forms of prostate cancer. However, little is known about the role of ANXA7 in the anticancer drug treatment towards different cancers.
The expression of ANXA7 was measured in the 60 cancer cell lines of the NCI-60 ADS project and correlated with the enhanced sensitivity to over 30,000 natural and synthetic compounds.
Eucalyptol showed a high positive correlation with ANXA7 expression and castration-resistant prostate cancer cell death occurred very effectively in response to the combination of eucalyptol and overexpressed wt-ANXA7 than either agent alone. The synergistic effects of ANXA7 and eucalyptol resulted in concordant changes in gene expression profiles particularly of Ras family members, MDM4, NF-ĸB and VEGF.
Overexpression of ANXA7 enhances eucalyptol cytotoxicity in prostate cancer cell lines.
背景/目的：我们的研究表明，膜联蛋白A7（ANXA7）是一种新型肿瘤抑制基因，在多种侵袭性前列腺癌中缺失。然而，关于ANXA7在针对不同癌症的抗癌药物治疗中的作用知之甚少。
在NCI-60药物筛选项目的60种癌细胞系中检测ANXA7的表达，并将其与对30000多种天然和合成化合物的敏感性增强相关联。
桉叶油素与ANXA7表达呈高度正相关，与单独使用任一药物相比，桉叶油素与野生型ANXA7过表达联合使用时，去势抵抗性前列腺癌细胞死亡非常有效。ANXA7和桉叶油素的协同作用导致基因表达谱发生一致变化，尤其是Ras家族成员、MDM4、核因子κB（NF-κB）和血管内皮生长因子（VEGF）。
ANXA7过表达增强了桉叶油素对前列腺癌细胞系的细胞毒性。
