Laboratory for Translational Cancer Research, Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi-221 005, India.
CSIR-National Institute for Interdisciplinary Science and Technology, Chemical Science and Technology Division, Organic Chemistry Section, Trivandrum-695019, India.
Biomolecules. 2019 Apr 23;9(4):159. doi: 10.3390/biom9040159.
Although over 100 species of Curcuma are reported, only is extensively studied. , a poorly studied species, is most commonly distributed in the Kerala state of India. For the first time, we examined the efficacy of different fractions (acetone, hexane, and ethyl acetate) of against glioma, cervical, and breast cancer cell lines. As determined by mitochondrial reductase activity assay, the viability of cancer cells was decreased in a concentration-dependent manner by the three fractions. The half maximal inhibitory concentration (IC-50) values after the treatment of C-6 glioma cells for 48 h was found to be 32.97 µg/mL (acetone extract), 40.63 µg/mL (hexane extract), and 51.65 µg/mL (ethyl acetate extract). Of the three fractions, the acetone fraction was more effective. The long-term colony formation of cancer cells was significantly suppressed by the acetone fraction. Analyses using DAPI (4',6-diamidino-2-phenylindole) staining, AO/PI (acridine orange/propidium iodide) staining, DNA laddering, and sub-G1 population revealed that the acetone extract induced apoptosis in glioma cells. The extract induced reactive oxygen species generation and suppressed the expression of cell survival proteins. The migration of cancer cells was also suppressed by the acetone extract. The gas chromatography-mass spectrometry (GC-MS) analysis indicated that tetracontane, dotriacontane, hexatriacontane, pentacosane, hexacosane, and eicosane are the major components in the acetone extract. Collectively, the extract from exhibited anti-carcinogenic activities in cancer cells. We are exploring whether the phytoconstituents, individually, or collectively contribute to the anti-cancer activities of .
尽管报道了超过 100 种姜黄属植物，但只有 被广泛研究。 是一种研究较少的物种，最常见于印度喀拉拉邦。我们首次研究了不同馏分（丙酮、己烷和乙酸乙酯）对神经胶质瘤、宫颈癌和乳腺癌细胞系的疗效。通过线粒体还原酶活性测定，三种馏分均以浓度依赖性方式降低癌细胞活力。用 C-6 神经胶质瘤细胞处理 48 h 后，半最大抑制浓度（IC50）值为 32.97 µg/mL（丙酮提取物）、40.63 µg/mL（己烷提取物）和 51.65 µg/mL（乙酸乙酯提取物）。在这三种馏分中，丙酮馏分最有效。长期集落形成实验表明，丙酮馏分显著抑制了癌细胞的集落形成。用 DAPI（4'，6-二脒基-2-苯基吲哚）染色、AO/PI（吖啶橙/碘化丙啶）染色、DNA 梯带和亚 G1 群体分析表明，丙酮提取物诱导神经胶质瘤细胞凋亡。提取物诱导活性氧生成并抑制细胞存活蛋白的表达。丙酮提取物还抑制了癌细胞的迁移。气相色谱-质谱（GC-MS）分析表明，在丙酮提取物中，二十四烷、三十二烷、三十六烷、二十五烷、二十六烷和二十烷是主要成分。总之，来自 的提取物在癌细胞中表现出抗癌活性。我们正在探索植物成分是否单独或共同导致 的抗癌活性。
